James P. McDonald Week 11

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Biological Terms

  1. Trehalose: "A crystalline disaccharide that is found in various organisms, is about half as sweet as sucrose, and is sometimes used as a sweetener in commercially prepared foods." [[1]]
  2. Batch Culture: "A large-scale closed system culture in which cells are grown in a fixed volume of nutrient culture medium under specific environmental conditions." [[2]]
  3. Transcriptome: "The complete set of RNA transcripts produced by the genome at any one time." [[3]]
  4. Ergosterol: "Crystalline steroid alcohol that occurs especially in yeast, molds, and ergot and is converted by ultraviolet irradiation ultimately into vitamin D2" [[4]]
  5. Cryostat: "An apparatus for maintaining a constant low temperature especially below 0°C" [[5]]
  6. Cuvette: "A transparent or translucent box-shaped container with precisely-measured dimensions for holding liquid samples to be put into a spectrophotometer." [[6]]
  7. Desaterase: "Any of several enzymes that putdouble bonds into the hydrocarbon areasof fatty acids." [[7]]
  8. Mannoproteins: "Yeast cell wall components that are proteins with large numbers of mannose groups attached; highly antigenic." [[8]]
  9. Prototrophic: "Strain's that have the same nutritional requirements as the wild-type strain." [[9]]
  10. Supernatant: "The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids." [[10]]

Outline

Introduction

  • Transcriptional regulation was studied in Saccharomyces cerevisiae in response to low temperatures within a chemostat.
    • This study focused on long-term low-temperature acclimation rather than rapid transitions to low temperature or "cold shock."
    • Transcription levels of various genes were measured at low temperatures.
  • The main result of the study revealed that there are large differences in transcription levels in many of the genes between the literature data, that looked at for rapid transitions to cold temperatures, and this study, that looked at long-term low-temperature acclimation.

Significance


Class Links

Journal Entries and Assignments

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