# James P. McDonald Week 8

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## Contents |

## Questions

### Discovery Questions from Chapter 4

5. on paper

6. At 1 hour X, Y, and Z are all black because they had ratios at 1. At 3 hours X, Y, and Z are all a dim red each with a ratio between 1.5-4.5x increase. At 5 hours X and Y go back to black because they are both around a ratio of 1. Z stays at a dim red with a 2x increase. At 9 hours X is a dim/medium green with about a 6x decrease, Y is bright green with about a 20x decrease and Z is still a dim red remaining at a 2x increase.

7. All three of the genes were transcribed similarly up to the 3 hour mark. They all started at a ratio of one and had similar increases. After 3 hours X and Y continued to behave similar at the 5 hour mark as they went back to a ratio around 1. At the 9 hour mark both the X and Y ratios decreased but the Y decreased a lot more than X. Gene Z did not behave anything like X and Y after the 3 hour mark.

9. Initially, the control and experimental cultures have an abundance of glucose so regulation remains constant. Therefore there is no change in the expression of the gene, resulting in the yellow spot. Later in the experiment the glucose is depleted and regulation changes.

10.

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**James P. McDonald Week 8**- James P. McDonald Week 9
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