Janelle N. Ruiz Assignment 13: Difference between revisions
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==Excel Files== | ==Filtered Excel Files== | ||
[[Media:IncHvsPgFold-Criterion0 FILTERED.xls|Increased H VS PG FOLD]] | [[Media:IncHvsPgFold-Criterion0 FILTERED.xls|Increased H VS PG FOLD]] |
Revision as of 11:43, 20 April 2010
Running GenMAPP
This began when i finished formatting my excel sheet and converting it into a Tab-Delimited txt file. After this I launched the GenMAPP program and loaded my Gene Database HS. This was followed by using the expression dataset menu and selecting my tab delimited file. After this GenMAPP began converting the raw expression data.
Our number of errors was 1792
The next step was labeling in the legend how we were corresponding our data into GenMAPP. Since we were working with 4 different folds: HvsPG, MetvsPG, HvsMet, and AIvsPG. This corresponded to 8 different criteria on the Expression dataset manager. Increased HvsPGfold >.25 AND HvsPGTtest <.05 Decreased HvsPGfold <-.25 AND HvsPGTtest <.05 Increased MetvsPGTest >.25 AND MetvsPG
Increased colors HvsPGfold inc=Red Opposite decreased Green MetvsPGfold inc=Brown Opposite decrease light blue HvsMetfold inc=Orange Opposite decreased light green AlVsPGfold inc=Purple Opposite blue
Grey no criteria
After this i ran MappFinder. Checking off "Gene Ontology" and "P value" as well as uploading the .gex file on the Lionshare website.
- Criterion 0 Increased H vs PG Fold
- Criterion 1Decreased H vs PG Fold
- Criterion 2 Increased Met vs PG Fold
- Criterion 3 Decreased Met vs Pg Fold
- Criterion 4 Increased H vs Met Fold
- Criterion 5 Decreased H vs Met fold
- Criterion 6 Increased AI vs PG fold
- Criterion 7 Decreased AI vs Pg fold
Top 10 Results
Criterion 0
- Regulation of retinal programmed cell death
- Neutrophil chemotaxi
- IgE binding
- Cell Surface
- Leukocye chemotaxis
- Defense response
- Peptidoglycan metabolic process
- Response to wounding
- MAPKKK Cascade
- Inflammatory response
Criterion 1
- Nucleosome
- Nucleosome assembly
- Chromatin assembly
- Chromatin assembly or dissaembly
- Cysteine biosynthetic process
- Protein carrier activity
- Protein DNA conplex assembly
- Chromatin
- Chromasomal part
- chromosome
Criterion 2
- M phase
- M phase of mitotic cell cycle
- Mitosis
- Cell Cycle phase
- Cell Division
- Mitotic Cell Cycle
- Spindle
- Cell Cycle Checkpoint
- Spindle organization and biogenesis
- Cell Cycle
Criterion 3
- Muscle contraction
- Extracellular region
- Sarcomere
- Extracellualr region part
- Contractile fiber part
- Regulation of muscle contraction
- Multicellular organismal process
- Monooxygenase activity
- Oxidoredcutase activity
- Anatomical structure development
Criterion 4
- Muscle contraction
- Multicellular organismal process
- Smooth muscle contraction
- Regulation of muscle contraction
- Anatomical structure development
- Extracellular region
- Multicellular organismal development
- Extracellular region part
- System development
- Regulation of multicellular organisms
Criterion 5
- Integral to plasma membrane
- Intrinsic to plasma membrane
- Cell communication
- Plasma membrane
- Plasma membrane part
- Signal transduction
- Receptor activity
- G-protein coupled receptor activity
- Molecular tranducer activity
- Signal transducer activity
Criterion 6
- M Phase
- Spindle pole
- Mitosis
- M Phase of Mitotic Cell Cycle
- Spindle
- Cell Cycle Phase
- Spindle Microtubule
- Mitotic cell cycle
- Microtuble
- MIcrotuble cytoskeleton
Criterion 7
- Startle response
- Glycine gated chloride channel activity
- Transmembrane receptor activity
- Synapse part
- Glycine binding
- Glycine gated ion channel activity
- Postsynaptic membrane
- Immune response
- Immunse system process
- Receptor activity
Filtered Excel Files
Class Links
Journal Assignments
- Shared Journal
- BIOL398-01/S10:Class Journal Week 2
- BIOL398-01/S10:Class Journal Week 3
- BIOL398-01/S10:Class Journal Week 4
- BIOL398-01/S10:Class Journal Week 5
- BIOL398-01/S10:Class Journal Week 6
- BIOL398-01/S10:Class Journal Week 7
- BIOL398-01/S10:Class Journal Week 8
- BIOL398-01/S10:Class Journal Week 9
- BIOL398-01/S10:Class Journal Week 11
- BIOL398-01/S10:Class Journal Week 12
- BIOL398-01/S10:Class Journal Week 13
- Assignments