Janelle N. Ruiz Assignment 2: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 17: Line 17:


*'''Question 3''': What features of the amino acid sequence make a protein a particular color?
*'''Question 3''': What features of the amino acid sequence make a protein a particular color?
The differences in color between different proteins depends upon a protein's amino acid sequences as explained above. The particular pigment which is reflected by a given protein will likely be determined by the amount of and interaction between aromatic amino acid side chains. Aromatic compounds, because of the alternating double and single bond ring system, absorb light and are often colored because of this. The size of the molecule (i.e. the amount of aromatic amino acid side chains) will often determine the color of the protein.  
The differences in color between different proteins depends upon a protein's amino acid sequences as explained above. The particular pigment which is reflected by a given protein will likely be determined by the amount of and interaction between aromatic amino acid side chains. Aromatic compounds, because of the alternating double and single bond ring system, absorb light and are colored because of this. The size of the molecule (i.e. the amount of aromatic amino acid side chains) and the structure of the aromatic side chain (i.e. the amount and order of the double and single bond ring system) will determine the color of the protein.  


*How do the colors combine to produce an overall color? How does this explain the genotype-phenotype rules you found in part (I)?
*'''Question 4''':How do the colors combine to produce an overall color? How does this explain the genotype-phenotype rules you found in part (I)?


#Which proteins are found in each of the four starting organisms?
 
#Using this knowledge, construct a purple protein.
 
#'''Question 5'''Which proteins are found in each of the four starting organisms?
 
#'''Question 6'''Using this knowledge, construct a purple protein.


back to [[User:Janelle N. Ruiz|Janelle N. Ruiz]]
back to [[User:Janelle N. Ruiz|Janelle N. Ruiz]]

Revision as of 22:04, 28 January 2010

Assignment II

Part II: Biochemistry

  • Question 1: What are the differences in the amino acid sequences of the proteins produced by the alleles you define in Part I? Hint: use the Compare menu to find the difference(s)between the amino acid sequences.

Allele Protein Structures

  • Green:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Tyr-Trp-Arg-Gln
  • Blue:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Tyr-Cys-Arg-Gln
  • Yellow:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Trp-Cys-Arg-Gln
  • Red:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Phe-Cys-Arg-Gln
  • White:Met-Ser-Asn-Arg-His-Ile-Leu-Leu-Val-Val-Cys-Arg-Gln

The differences in the amino acid sequences are found at position 10 and 11.

  • Question 2: What features of the amino acid sequence make a protein pigmented?

The properties of amino acid side chains make a protein pigmented as well as the sequence of the amino acids which make up the protein. How a protein is structured (i.e. its amino acid sequence) will ultimately determine how and to what degree it is able to reflect and absorb specific wavelengths of light and therefore its percieved pigment.

  • Question 3: What features of the amino acid sequence make a protein a particular color?

The differences in color between different proteins depends upon a protein's amino acid sequences as explained above. The particular pigment which is reflected by a given protein will likely be determined by the amount of and interaction between aromatic amino acid side chains. Aromatic compounds, because of the alternating double and single bond ring system, absorb light and are colored because of this. The size of the molecule (i.e. the amount of aromatic amino acid side chains) and the structure of the aromatic side chain (i.e. the amount and order of the double and single bond ring system) will determine the color of the protein.

  • Question 4:How do the colors combine to produce an overall color? How does this explain the genotype-phenotype rules you found in part (I)?


  1. Question 5Which proteins are found in each of the four starting organisms?
  1. Question 6Using this knowledge, construct a purple protein.

back to Janelle N. Ruiz

Links

BIOL398-01/S10:Week 2

Notes

Format

  • allele
  • color
  • amino acid sequence (highlight differences)