Janet B. Matsen:Closed Lab Questions: Difference between revisions
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**A: It probably means you overloaded the PCR with too many cells. | **A: It probably means you overloaded the PCR with too many cells. | ||
==My primers are yellow!== | ==Primers/Oligos== | ||
===Should I pay extra to further purify large bp oligos?=== | |||
*Usually no. The sequencing companies just want your money. Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad. | |||
===My primers are yellow!=== | |||
*Don't worry -- this is normal! [[Image:Yellow_primers.jpg|thumb|normal oligos have a yellow color that is visible with large synthesis scales]] | *Don't worry -- this is normal! [[Image:Yellow_primers.jpg|thumb|normal oligos have a yellow color that is visible with large synthesis scales]] | ||
Revision as of 21:31, 23 February 2012
Back to Janet
I define "closed" as "not bothering me incessantly." One can always learn more but we have to stop at a point otherwise we would never move forward with our research!
If you have any input/corrections/references please drop [User:Janet B. Matsen|me] a line.
Colony PCR
- Q: The gel is dark where I loaded it -- what does this mean?
- A: It probably means you overloaded the PCR with too many cells.
Primers/Oligos
Should I pay extra to further purify large bp oligos?
- Usually no. The sequencing companies just want your money. Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad.
My primers are yellow!
- Don't worry -- this is normal!
normal oligos have a yellow color that is visible with large synthesis scales
