Janet B. Matsen:Closed Lab Questions
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I define "closed" as "not bothering me incessantly." One can always learn more but we have to stop at a point otherwise we would never move forward with our research!
If you have any input/corrections/references please drop [User:Janet B. Matsen|me] a line.
Colony PCR
- Q: The gel is dark where I loaded it -- what does this mean?
- A: It probably means you overloaded the PCR with too many cells.
Primers/Oligos
Should I pay extra to further purify large bp oligos?
- Usually no. The sequencing companies just want your money. Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad.
My primers are yellow!
- Don't worry -- this is normal!
normal oligos have a yellow color that is visible with large synthesis scales
