Janet B. Matsen:Growing Cultures For Crude Extract
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growing culture
- streak fresh plates from freezer stock
- inoculate overnight cultures for subsequent inoculation
- inoculate into 100 mL of M9 media
- grow to mid-exponential at 37oC, shaking at 225 rpm
- add inducers (aTc & IPTG) and shake at 18oC & 200 rpm for 36 hours!
storing cells
- pour into 2 50 mL flasks
- centrifuge for ___ min at ___ rpm
- flash freeze with liquid N2
- store @ -20oC