Janet B. Matsen:Guide to Gibson Assembly: Revision history

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curprev 13:2813:28, 23 September 2019‎ Janet B. Matsen talk contribs‎ 31,083 bytes +29‎ No edit summary
curprev 13:2513:25, 23 September 2019‎ Janet B. Matsen talk contribs‎ 31,054 bytes +221‎ No edit summary

curprev 13:2413:24, 2 October 2015‎ Janet B. Matsen talk contribs‎ 30,833 bytes +174‎ →‎Other Resources

curprev 15:4515:45, 6 June 2015‎ Janet B. Matsen talk contribs‎ 29,773 bytes +366‎ →‎Transformation
curprev 15:4415:44, 6 June 2015‎ Janet B. Matsen talk contribs‎ 29,407 bytes +207‎ →‎Transformation

curprev 09:4909:49, 30 April 2014‎ Janet B. Matsen talk contribs‎ 29,200 bytes +9‎ →‎Other Resources
curprev 09:4909:49, 30 April 2014‎ Janet B. Matsen talk contribs‎ 29,191 bytes −46‎ →‎Other Resources
curprev 09:4809:48, 30 April 2014‎ Janet B. Matsen talk contribs‎ 29,237 bytes +377‎ →‎Other Resources
curprev 09:4709:47, 30 April 2014‎ Janet B. Matsen talk contribs‎ 28,860 bytes −19‎ →‎Consolidated Version of Protocol

curprev 07:5307:53, 6 April 2014‎ Janet B. Matsen talk contribs‎ 28,879 bytes 0‎ →‎Thanks
curprev 07:5207:52, 6 April 2014‎ Janet B. Matsen talk contribs‎ 28,879 bytes +516‎ →‎Transformation

curprev 11:3011:30, 19 March 2014‎ Janet B. Matsen talk contribs‎ 28,363 bytes +68‎ →‎Screening

curprev 13:3513:35, 18 March 2014‎ Janet B. Matsen talk contribs‎ 28,295 bytes +398‎ →‎Time saving tips
curprev 13:3013:30, 18 March 2014‎ Janet B. Matsen talk contribs‎ 27,897 bytes +1,882‎ →‎Time saving tips

curprev 17:0117:01, 5 March 2014‎ Janet B. Matsen talk contribs‎ 26,015 bytes +35‎ →‎At the assembly step
curprev 16:5316:53, 5 March 2014‎ Janet B. Matsen talk contribs‎ 25,980 bytes +34‎ →‎Thanks
curprev 16:5316:53, 5 March 2014‎ Janet B. Matsen talk contribs‎ 25,946 bytes +201‎ →‎Making your own Gibson mix

curprev 13:0713:07, 13 February 2014‎ Janet B. Matsen talk contribs‎ 25,745 bytes +5‎ →‎Transformation
curprev 13:0613:06, 13 February 2014‎ Janet B. Matsen talk contribs‎ 25,740 bytes +179‎ →‎Transformation

curprev 18:2418:24, 12 February 2014‎ Janet B. Matsen talk contribs‎ 25,561 bytes +13‎ →‎Transformation
curprev 18:2318:23, 12 February 2014‎ Janet B. Matsen talk contribs‎ 25,548 bytes +241‎ →‎Transformation

curprev 15:4715:47, 11 February 2014‎ Janet B. Matsen talk contribs‎ 25,307 bytes +124‎ →‎Transformation

curprev 06:3306:33, 29 January 2014‎ Janet B. Matsen talk contribs‎ 25,183 bytes +1‎ No edit summary
curprev 06:1906:19, 29 January 2014‎ Janet B. Matsen talk contribs‎ 25,182 bytes +1‎ →‎Gibson assembly reaction
curprev 06:1906:19, 29 January 2014‎ Janet B. Matsen talk contribs‎ 25,181 bytes +12‎ →‎Gibson assembly reaction

curprev 15:2415:24, 6 January 2014‎ Janet B. Matsen talk contribs‎ 25,169 bytes +196‎ →‎At the assembly step
curprev 15:0915:09, 6 January 2014‎ Janet B. Matsen talk contribs‎ 24,973 bytes +13‎ →‎Consolidated Version of Protocol
curprev 15:0515:05, 6 January 2014‎ Janet B. Matsen talk contribs‎ 24,960 bytes −1‎ →‎Nano Drop PCR fragments

curprev 08:2008:20, 21 November 2013‎ Janet B. Matsen talk contribs‎ 24,151 bytes +133‎ →‎Intro
curprev 08:1908:19, 21 November 2013‎ Janet B. Matsen talk contribs‎ 24,018 bytes +511‎ →‎Generate PCR fragments

curprev 08:4608:46, 18 November 2013‎ Janet B. Matsen talk contribs‎ 23,507 bytes +135‎ →‎Design primers

curprev 08:0108:01, 25 October 2013‎ Janet B. Matsen talk contribs‎ 23,372 bytes +212‎ →‎Intro

curprev 07:2307:23, 17 October 2013‎ Janet B. Matsen talk contribs‎ 22,851 bytes +1,175‎ →‎Screening
curprev 07:1307:13, 17 October 2013‎ Janet B. Matsen talk contribs‎ 21,676 bytes +146‎ →‎Screening

curprev 06:2706:27, 15 October 2013‎ Janet B. Matsen talk contribs‎ 21,530 bytes +2,289‎ →‎Screening
curprev 06:0506:05, 15 October 2013‎ Janet B. Matsen talk contribs‎ 19,241 bytes +270‎ →‎Transformation

curprev 06:4306:43, 17 September 2013‎ Janet B. Matsen talk contribs‎ 13,473 bytes −54‎ →‎Prepare plasmid maps
curprev 06:4006:40, 17 September 2013‎ Janet B. Matsen talk contribs‎ 13,527 bytes +110‎ →‎Prepare plasmid maps
curprev 06:3906:39, 17 September 2013‎ Janet B. Matsen talk contribs‎ 13,417 bytes +78‎ No edit summary

curprev 13:1613:16, 22 March 2013‎ Janet B. Matsen talk contribs‎ 13,339 bytes +29‎ →‎Other Resources
curprev 13:1613:16, 22 March 2013‎ Janet B. Matsen talk contribs‎ 13,310 bytes +648‎ →‎Other Resources

curprev 09:2309:23, 13 March 2013‎ Janet B. Matsen talk contribs‎ 12,662 bytes +569‎ →‎Generate PCR fragments
curprev 09:1809:18, 13 March 2013‎ Janet B. Matsen talk contribs‎ 12,093 bytes +204‎ →‎Generate PCR fragments

curprev 14:5614:56, 8 March 2013‎ Janet B. Matsen talk contribs‎ 11,889 bytes +359‎ →‎Examples

curprev 16:5816:58, 27 February 2013‎ Janet B. Matsen talk contribs‎ 11,530 bytes +263‎ →‎Screening

curprev 15:3415:34, 25 February 2013‎ Janet B. Matsen talk contribs‎ 11,267 bytes +124‎ →‎Tricky Cases

curprev 17:4417:44, 20 February 2013‎ Janet B. Matsen talk contribs‎ 11,143 bytes +60‎ No edit summary

curprev 12:5212:52, 5 October 2012‎ Janet B. Matsen talk contribs‎ 112 bytes +112‎ New page: * you can chose where the seam is if you use longer oligos * Dpn1 * RFP for backbone: don't screen red colonies!

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