Janet B. Matsen:Key Ideas: Difference between revisions
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(New page: Back to Janet Notes for my project by date: *2012/05: Do we have an easy (non enzymatic) assay for methanol? Betsy's Answer: Not that I'm aware of but you could ...) |
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*2012/05: Do we have an easy (non enzymatic) assay for methanol? Betsy's Answer: Not that I'm aware of but you could probably come up with a bioassay using feeding experiments if it doesn't have to be quantitative. What are you trying to do? | *2012/05: Do we have an easy (non enzymatic) assay for methanol? Betsy's Answer: Not that I'm aware of but you could probably come up with a bioassay using feeding experiments if it doesn't have to be quantitative. What are you trying to do? | ||
*2012/04: You can drive a few kb with LacI: naturally it drives ~4.9 kb (lacZ = 3075, lacY: 1254 bp, lacA: 612 bp) | *2012/04: You can drive a few kb with LacI: naturally it drives ~4.9 kb (lacZ = 3075, lacY: 1254 bp, lacA: 612 bp) | ||
*2012/08: when you have cells under starvation, it can look like they grow for two reasons: | |||
**1. they start eating the proteins in the ribosomes | |||
**2. they can divide, which increases OD | |||
**note: neither of these cause increases in dry cell weight, so this is a good way to measure growth. | |||
Revision as of 10:20, 28 August 2012
Back to Janet
Notes for my project by date:
- 2012/05: Do we have an easy (non enzymatic) assay for methanol? Betsy's Answer: Not that I'm aware of but you could probably come up with a bioassay using feeding experiments if it doesn't have to be quantitative. What are you trying to do?
- 2012/04: You can drive a few kb with LacI: naturally it drives ~4.9 kb (lacZ = 3075, lacY: 1254 bp, lacA: 612 bp)
- 2012/08: when you have cells under starvation, it can look like they grow for two reasons:
- 1. they start eating the proteins in the ribosomes
- 2. they can divide, which increases OD
- note: neither of these cause increases in dry cell weight, so this is a good way to measure growth.
