1. 'Adaptive mistranslation'
2. Mycobacteria and host-pathogen interactions
3. Protein translation fidelity
4. GatCAB dependent tRNA synthesis and protein evolution
The Javid lab principal interest is in mycobacterial pathophysiology. Tuberculosis causes more infectious disease deaths in China than any other pathogen, and the basic biology of its causative organism,Mycobacterium tuberculosis is still poorly understood.Our lab is interested in how fundamental physiological processes in mycobacteria can influence important clinical phenotypes such as antibiotic tolerance and immune evasion. We have shown that altering the protein translation error rate in mycobacteria can profoundly influence phenotypic resistance to first-line anti-mycobacterial drugs. We are now trying to understand the molecular mechanism and regulation of translational fidelity.Mycobacterium tuberculosis can reside within the host for decades without detection. We are trying to understand how this happens -- at the level of both innate and adaptive immunity.
Bio_luminescence by Nano Luciferase (Promega), which we optimised to be easily expressed in Mycobacterium, and a novel mistranslation reporter with 100 times higher signal over canonical Renilla luciferase.