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/ Home / NEWS / Research / Publications / Lab Members / Lab Alumni / Fun Stuff / Contact / PI Babak Javid Babak Javid is a physician scientist with an interest in tuberculosis, infectious diseases and protein translational fidelity. He did his medical training in the UK, his PhD on antigen processing and presentation with Paul Lehner at Cambridge, and post-doctoral training on mycobacterial genetics with Eric Rubin at Harvard. He joined Tsinghua School of Medicine in October 2011. Email: bjavid{at}gmail.com (Please replace {at} to @ in the address.) Postdocs Melody Toosky Post-doc, Tsinghua University, 2012- present; Ph.D., Microbiology and Immunology, University of North Dakota 2012; B.S., Biology, University of California, Irvine 2004; Hobbies: Chinese language, culture, music and food Email: mtoosky{at}gmail.com (Please replace {at} to @ in the address.) Mycobacterial infections are characterized by the need of the host to contain the invading pathogen as well as by the need of the pathogen to escape the host cell in order to infect other cells. An important phase during this interaction pertains to host cell death decision pathway. My project involves looking at this pathway using proteomic tools to get some clues about how mycobacteria can influence the manner in which host cells die.; Graduate Students Zhu, Junhao 朱军豪 Third-year Postgraduate Student (PTN Programme) 2011.09-present: College of Life Science, Peking University, PhD; 2007.09-2011.07: College of Life Science, Peking University ,Bachelor; Hobbies: basketball, painting and cooking E-mail address: newforlofe{at}gmail.com (Please replace {at} to @ in the address.) In a homogeneous bacterial population (identical in genomic information), the phenotypes of individual cells may vary significantly due to gene expression, protein synthesis and other processes. One reason for this variation may be the rate of errors generated during protein synthesis (mistranslation), which we hypothesise may benefit cell survival when they are challenged by stress or antibiotics. I am designing novel reporters to measure mistranslation at the single cell level. I hope to find what's controlling the heterogeneity of mistranslation in a clonal population and a potential clues for novel drug development. Su, Hongwei 苏宏伟 Second-year Postgraduate Student (PTN Programme) 2012.09-present: College of Life Science, Tsinghua University, PhD; 2007.09-2012.07: College of Animal Husbandry and Veterinary, Jilin university, Bachelor; Hobbies: basketball, and other sports, watching movies Email: suhongwei0108{at}gmail.com (Please replace {at} to @ in the address.) Based on previous work of the lab, mycobacteria could tolerate high mistranslation rates and this phenotype could help them to adapt to different kinds of environment including some antibiotic such as rifampicin. So there may be some specific elements which control translational fidelity. My project is to find the genetic elements controlling mycobacterial translation fidelity using forward genetic screens. Cai, Rongjun 蔡荣俊 Second-year Postgraduate Student 2012.09-present: School of Medicine, Tsinghua University, PhD; 2008.09-2012.07: College of Animal Sciences & Technology, Huazhong Agricultural University, Bachelor; Hobbies: computer techniques Email: kim32000{at}hotmail.com (Please replace {at} to @ in the address.) In mycobacteria tRNAAsn and tRNAGln are generated by a two-step synthesis, involving physiological misacylation to Glu-tRNAGln and Asp-tRNAAsn intermediates which are amidated to the cognate forms by GatCAB. I am focusing on the role of GatCAB in this indirect pathway to identify its potential link to relative mistranslation rate in mycobacteria. I am also interested in mechanisms to manipulate mycobacteria genetically including generation of SNPs via recombineering. Long, Jing 龙晶 Second-year Postgraduate Student 2012.09-present: School of Medicine, Tsinghua University, PhD; Hobbies: photography, food My projects are based on the hypothesis that mistranslation may contribute to mycobacterial adaption to the intracellular environment. One of my projects is to verify the hypothesis by measuring the mycobacterial mistranslation rate before or after infection; the other one is to understand the interactions between the host and mycobacteria during the process of infection. Li, Hao 李浩 First-year Postgraduate Student (PTN Programme) 2013.09-present: College of Life Science, Tsinghua University, PhD; 2011.07-2013.09: School of medicine, Tsinghua University, Research assistant; 2008.09-2011.07: Academy of Military Medical Sciences, Master degree; 2004.08-2008.8: Henan Agricultural University, Bachelor degree; Hobbies: basketball, hiking, movies E-mail address: leehao{at}biomed.tsinghua.edu.cn (Please replace {at} to @ in the address.) The main Research fields: 1)The interaction of Mycobacteria tuberculosis with the host. About one third of the population in the world is infected with Mtb and remain asymptomatic. Of the latent population, about 5% will develop active tuberculosis in their lifetime. We want to know that why some individuals are protected from infection while others not. 2)The mechanisms of drug resistance and tolerance in Mycobacteria. The MDX/XDR has become a big problem in the world especially in China. Also the Mycobacteria become drug tolerance via natural selection. These may be big barriers to shortening TB treatment. We want to find the mechanisms behind these problems. Chen, Yanan 陈亚男 First-year Postgraduate Student (PTN Programme) 2013.09-present: College of Life Science, Tsinghua University, PhD; 2009.09-2013.07: College of Life Science, Nanjing Agricultural University, Bachelor; Hobbies: Korean language, reading novels Email: chenyanan90618{at}163.com (Please replace {at} to @ in the address.) I am investigating the role of EF-Tu discrimination in mycobacterial mistranslation, which will examine one potential mechanism for mycobacterial mistranslation. The residing dogma states that misacylated tRNAs cannot take part in protein translation due to discrimination by EF-Tu, but this has not been investigated in vivo. We know that fidelity in translation is associated with two key events: synthesis of correctly charged aminoacyl transfer RNAs (aa-tRNAs) by aminoacyl tRNA synthetases (aaRSs) and accurate selection of aa-tRNAs by the ribosome. However, the precise molecular mechanism of mycobacterial mistranslation is poorly understood. Specifically, it is not known if mistranslation results from errors of ribosomal decoding or misacylation of amino-acylated tRNAs. This is particularly relevant since mycobacteria physiologically misacylate glutamine and asparagine tRNAs before modifying them to the correct form by the action of the enzyme GatCAB. Zhang,Yiwei 张一薇 First-year Postgraduate Student 2013.09-present: College of Medicine, Tsinghua University, PhD; 2009.09-2013.07: College of Life Science, Nanjing Agricultural University, Bachelor; Hobbies: Violin, travelling Email: zhang-yw13{at}mails.tsinghua.edu.cn (Please replace {at} to @ in the address.) The previous work of the lab indicates that mistranslation may help mycobacteria survive in some antibiotic environments such as rifampicin. This finding leads us to further think the mechanism and effects of mistranslation in eukaryote. So, I choose yeast as my research material, trying to find the genetic elements controlling translation fidelity and find out what effects mistranslation may have on eukaryote. Chen,Yuxiang 陈宇翔 First-year Postgraduate Student 2013.09-present: School of Life Science, Tsinghua University, PhD; 2009.09-2013.06: School of Life Science, Sichuan University, Bachelor; Hobbies: Music, language, Computer games Email: chenyx13{at}mails.tsinghua.edu.cn (Please replace {at} to @ in the address.) My research mainly focuses on the biochemistry of kasugamyin. It has been reported that kasugamycin may increase translation fidelity but little is known about its molecular mechanism. And in E.coli kasugamycin can induce a special ribosome called the 61S ribosome, which specially translate lmRNA. In mycobacterial tuberculosis, nearly 26% of the mRNA is leaderless. My goal is to find out the relationship between these aspects. Research Staff Duan,Lei 段蕾 Assistant / Lab manger 2013.08-present: School of medicine,Tsinghua University, Research assistant; 2010.09-2010.07: Shanxi medical University, Master degree; 2005.09-2010.07: Lanzhou University, Bachelor degree; Hobbies: reading, cooking, music Email: duanlei87{at}126.com (Please replace {at} to @ in the address.) As lab manager, I ensure the smooth and efficient running of the lab. I also act as Babak's assistant and administrative aid. Pan, Miaomiao 潘苗苗 Research Assistant 2012.07-present: School of medicine, Tsinghua University, Research assistant; 2009.09-2012.07: Nankai University, Master degree; 2005.09-2009.07: Shaanxi University of Technology, Bachelor degree. Hobbies: reading, watching movies, climbing and sleeping E-mail address: panmm99{at}gmail.com (Please replace {at} to @ in the address.) I am interested in the hypothesis that a high phenotypic drug resistance in mycobacteria species is caused by high adaptive mistranslation. I am currently developing a gain of function reporter to measure the mistranslation rate using a M. smegmatis model. I will use this reporter to screen for molecules that alter mycobacterial translational fidelity. Wen Kawaji 雯雯 Research Assistant 2013-present: School of medicine, Tsinghua University, Research assistant; 2011-2013: Johns Hopkins University School of Public Health, Master of Science; 2006-2011: Johns Hopkins University, Bachelor degree; Hobbies: Food, books, films, and travel E-mail address: wenkawaji{at}gmail.com (Please replace {at} to @ in the address.) The Main research field: Small molecule screening. Mistranslation is important and necessary for mTB phenotypic resistance. Through small molecule screening, we are looking for potential drugs that would play a role in translation fidelity. Undergraduate Students Leng, Tianqi 冷天祺 Senior Undergraduate Student (Peking University) Hobbies: Marvel superhero movies, swimming, table tennis and badminton. Email: lengtq{at}gmail.com (Please replace {at} to @ in the address.) My project investigates the repertoire and rate of mycobacterial mistranslation in the model organism Mycobacterium smegmatis. I will use gain of function luminescence reporters to accurately identify the rate of many different types of translational error. He, Qiao 贺桥 Senior Undergraduate Student (Peking University) Hobbies: Surfing Internet, playing basketball, reading Email: rodefo{at}gmail.com (Please replace {at} to @ in the address.) I am interested in using fluorescent reporters to measure mycobacterial mistranslation rates. Wang, Xun 王珣 Junior Undergraduate Student Hobbies: Reading, swimming, violin performance, Chinese cooking Email: wangxun1027{at}gmail.com (Please replace {at} to @ in the address.) Wu, You 吴悠 Junior Undergraduate Student, China Agricultural University Hobbies: Watercolor painting; Fruit; Rilakkuma Email: vittie974{at}163.com (Please replace {at} to @ in the address.)

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