Jessica Karen Wong/Notebook/2007-8-3

• Sent for sequencing
  • I2056 scarred in 3K3 colonies 4-7, I2057-ES and I2057-EX sealed w/ Not1
  • Each processed with VF and VR primers
• Started Mutagenesis
  • Did thermal cycling and digests
  • Waiting to transform until we are sure we have competent cells
• Saw no growth on any of the 10 overnight plates - PCR I2055 sealed and with RBS's, and PCR I2057 with Promoters
• Replated all of yesterday's transformations in Felix's new batch of Top10
• Digested presealed I2057-ES and I2057-EX E/S and E/X respectively
  • Used both DNA in EB and in 8.5 water