Jessica Karen Wong/Notebook/2007-8-9: Difference between revisions
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**I2056 (Scar) colony 7 to redo | **I2056 (Scar) colony 7 to redo | ||
[[Image:8-9.jpg|thumb|left|I2056 Colony PCR Gel]] | |||
*Colony PCRed sealed I2056 8 of each ENX and SNX | *Colony PCRed sealed I2056 8 of each ENX and SNX | ||
**Ran on gel loaded I2056-ENX (1-8) sp lad sp I2056-SNX (1-8) | **Ran on gel loaded I2056-ENX (1-8) sp lad sp I2056-SNX (1-8) | ||
**Top row was PCR's w/ too dilute primers, bottom was normal (exact same cell samples loaded same order) | **Top row was PCR's w/ too dilute primers, bottom was normal (exact same cell samples loaded same order) | ||
Revision as of 13:30, 10 August 2007
- All overnight plates (I2056's and I2055's from yesterday's ligations) have a good amount of growth, very little fluorescence though
- However, last time they got a lot more red after being in the fridge for a night
- Will check on them again tomorrow and then make overnights of bright colonies
- Minipreped overnight cultures (B0032 w/ EB, rest w/ 8.5 water)
- Sent for sequencing, each with VF and VR
- I2055-ENX colony 2
- I2055-SNX (colonies 6 and 7)
- I2055-EX-B0032
- I2057 sealed EX and ES to redo
- I2056 (Scar) colony 7 to redo
- Colony PCRed sealed I2056 8 of each ENX and SNX
- Ran on gel loaded I2056-ENX (1-8) sp lad sp I2056-SNX (1-8)
- Top row was PCR's w/ too dilute primers, bottom was normal (exact same cell samples loaded same order)
