Jessica Keenan: Biomaterials Engineering: Difference between revisions
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(New page: =For Next Time Day 1= ==A Synthetic Gene Network for Tuning Protein Degradation in Saccharomyces Cerevisiae== *Grilly C, Stricker J, Pang WL, Bennett MR, Hasty J. A synthetic gene network...) |
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*Grilly C, Stricker J, Pang WL, Bennett MR, Hasty J. A synthetic gene network for tuning protein degradation in Saccharomyces cerevisiae . Molecular Systems Biology 2007; 3:127 | *Grilly C, Stricker J, Pang WL, Bennett MR, Hasty J. A synthetic gene network for tuning protein degradation in Saccharomyces cerevisiae . Molecular Systems Biology 2007; 3:127 | ||
*The authors of this article created a mechanism in yeast that uses IPTG to tune the degradation levels of proteins tagged with a specific amino acid sequence. They have also produced a predictive model for their system, making it even more useful. It would be interesting to see if this system (or a modified version of this system) could be made to work in mammalian cells. Eventually, such a system could be used to degrade proteins over-expressed in cancers (for example, the Her receptors in breast cancer). | *The authors of this article created a mechanism in yeast that uses IPTG to tune the degradation levels of proteins tagged with a specific amino acid sequence. They have also produced a predictive model for their system, making it even more useful. It would be interesting to see if this system (or a modified version of this system) could be made to work in mammalian cells. Eventually, such a system could be used to degrade proteins over-expressed in cancers (for example, the Her receptors in breast cancer). | ||
==Bypass of DNA Lesions Generated During Anticancer Treatment with Cisplatin by DNA Polymerase η== | |||
*Alt A, Lammens K, Chiocchini C, Lammens A, Pieck JC, Kuch D, Hopfner KP, Carell T. Bypass of DNA lesions generated during anticancer treatment with cisplatin by DNA Polymerase η. Science 2007; 318:967-970 | |||
*Here the authors describe how a DNA polymerase is able to replicate DNA even though it has been damaged by chemotherapy. Since the cell continues to replicate its mutated genome instead of dying, it is resistant to the the therapy. RNAi could be used to study how the depletion of this gene is able to alter cancer growth. | |||
Revision as of 16:13, 12 November 2007
For Next Time Day 1
A Synthetic Gene Network for Tuning Protein Degradation in Saccharomyces Cerevisiae
- Grilly C, Stricker J, Pang WL, Bennett MR, Hasty J. A synthetic gene network for tuning protein degradation in Saccharomyces cerevisiae . Molecular Systems Biology 2007; 3:127
- The authors of this article created a mechanism in yeast that uses IPTG to tune the degradation levels of proteins tagged with a specific amino acid sequence. They have also produced a predictive model for their system, making it even more useful. It would be interesting to see if this system (or a modified version of this system) could be made to work in mammalian cells. Eventually, such a system could be used to degrade proteins over-expressed in cancers (for example, the Her receptors in breast cancer).
Bypass of DNA Lesions Generated During Anticancer Treatment with Cisplatin by DNA Polymerase η
- Alt A, Lammens K, Chiocchini C, Lammens A, Pieck JC, Kuch D, Hopfner KP, Carell T. Bypass of DNA lesions generated during anticancer treatment with cisplatin by DNA Polymerase η. Science 2007; 318:967-970
- Here the authors describe how a DNA polymerase is able to replicate DNA even though it has been damaged by chemotherapy. Since the cell continues to replicate its mutated genome instead of dying, it is resistant to the the therapy. RNAi could be used to study how the depletion of this gene is able to alter cancer growth.
