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Revision as of 21:00, 15 July 2008 by Jhdavis (Talk | contribs)
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  1. Transform pCP20 (p202 for me) - this is ampR and ts - grow at 30C
  2. grow colony at 30, then streak out on LB only plate, plate at 42
  3. pick colony, grow up and streak out on lb only, lb/amp, lb/markertrying to remove, plate at 30.
  4. save clone if it shows correct phenotype
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