Jhdavis::GelFiltrationStandards: Difference between revisions
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http://www5.gelifesciences.com/aptrix/upp00919.nsf/Content/6EEE47990D9F933EC1256F90000DD697/$file/18102218AJ.pdf | |||
'HiPrep 16/60 Sephacryl s-100 HR'' | 'HiPrep 16/60 Sephacryl s-100 HR'' | ||
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Cytidine | Cytidine | ||
[[Image:GFstandards.jpg]] | |||
TECHNICAL SPECIFICATIONS | |||
Superdex™ prep grade | |||
Fractionation range (Mr) | |||
- Globular proteins | |||
Superdex™ 30 prep grade < 1 × 104 | |||
Superdex™ 75 prep grade 3 × 103-7 × 104 | |||
Superdex™ 200 prep grade 1 × 104-6 × 105 | |||
- Dextrans | |||
Superdex™ 75 prep grade 5 × 102-3 × 104 | |||
Superdex™ 200 prep grade 1 × 103-1 × 105 | |||
Matrix Spherical composite of cross-linked | |||
agarose and dextran | |||
Average particle size 34 µm | |||
Chemical stability Stable in all common buffers: | |||
1 M acetic acid, 8 M urea, | |||
6 M guanidine hydrochloride, | |||
30% isopropanol, 70% ethanol, | |||
1 M NaOH (for cleaning-in-place) | |||
pH stability 3-12 (working and long term) | |||
1-14 (short term) | |||
Storage 20% ethanol | |||
Storage temperature 4°C to 30°C | |||
Prepacked HiLoad™ Columns | |||
Bed volume HiLoad™ 16/60: 120 ml | |||
HiLoad™ 26/60: 320 ml | |||
Sample volume HiLoad™ 16/60: < 5 ml | |||
HiLoad™ 26/60: < 13 ml | |||
Linear flow rate 10-50 cm/h | |||
Max. pressure | |||
Over the packed bed during operation: | |||
3 bar (42 psi, 0.3 MPa) | |||
Column hardware: 5 bar (73 psi, 0.5 MPa) | |||
Theoretical plates > 13 000 m-1 |
Latest revision as of 04:24, 8 July 2008
'HiPrep 16/60 Sephacryl s-100 HR
Void: 38 mL
IgG 40
BSA 47
B-lactoglobin 56
Cytochrome C 70
Cytidine 120
HiPrep 16/60 Sephacryl S-200 HR
Void: 40 mL
IgG 49
BSA 59
B-lactoglobin 64
Cytochrome C 78
Cytidine 120
HiPrep 16/60 Sephacryl S-300 HR
Void: 60 mL
IgG 71.3
BSA 77.5
B-lactoglobin 82
Cytochrome C 93.8
Cytidine 122.3
MW:
IgG 160,000
BSA 67,000
B-lactoglobin 35,000
Cytochrome C 12,400
Cytidine 240
Rg:
IgG 5.7504
BSA 3
B-lactoglobin 1.8
Cytochrome C 1.39
Cytidine
TECHNICAL SPECIFICATIONS Superdex™ prep grade Fractionation range (Mr) - Globular proteins Superdex™ 30 prep grade < 1 × 104 Superdex™ 75 prep grade 3 × 103-7 × 104 Superdex™ 200 prep grade 1 × 104-6 × 105 - Dextrans Superdex™ 75 prep grade 5 × 102-3 × 104 Superdex™ 200 prep grade 1 × 103-1 × 105 Matrix Spherical composite of cross-linked agarose and dextran Average particle size 34 µm Chemical stability Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning-in-place) pH stability 3-12 (working and long term)
1-14 (short term)
Storage 20% ethanol Storage temperature 4°C to 30°C Prepacked HiLoad™ Columns Bed volume HiLoad™ 16/60: 120 ml HiLoad™ 26/60: 320 ml Sample volume HiLoad™ 16/60: < 5 ml HiLoad™ 26/60: < 13 ml Linear flow rate 10-50 cm/h Max. pressure Over the packed bed during operation: 3 bar (42 psi, 0.3 MPa) Column hardware: 5 bar (73 psi, 0.5 MPa) Theoretical plates > 13 000 m-1