Jhdavis::GelFiltrationStandards

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http://www5.gelifesciences.com/aptrix/upp00919.nsf/Content/6EEE47990D9F933EC1256F90000DD697/$file/18102218AJ.pdf

'HiPrep 16/60 Sephacryl s-100 HR

Void: 38 mL

IgG 40

BSA 47

B-lactoglobin 56

Cytochrome C 70

Cytidine 120

HiPrep 16/60 Sephacryl S-200 HR

Void: 40 mL

IgG 49

BSA 59

B-lactoglobin 64

Cytochrome C 78

Cytidine 120

HiPrep 16/60 Sephacryl S-300 HR

Void: 60 mL

IgG 71.3

BSA 77.5

B-lactoglobin 82

Cytochrome C 93.8

Cytidine 122.3

MW:

IgG 160,000

BSA 67,000

B-lactoglobin 35,000

Cytochrome C 12,400

Cytidine 240

Rg:

IgG 5.7504

BSA 3

B-lactoglobin 1.8

Cytochrome C 1.39

Cytidine

Image:GFstandards.jpg

TECHNICAL SPECIFICATIONS Superdex™ prep grade Fractionation range (Mr) - Globular proteins Superdex™ 30 prep grade < 1 × 104 Superdex™ 75 prep grade 3 × 103-7 × 104 Superdex™ 200 prep grade 1 × 104-6 × 105 - Dextrans Superdex™ 75 prep grade 5 × 102-3 × 104 Superdex™ 200 prep grade 1 × 103-1 × 105 Matrix Spherical composite of cross-linked agarose and dextran Average particle size 34 µm Chemical stability Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning-in-place) pH stability 3-12 (working and long term)

	1-14 (short term) 

Storage 20% ethanol Storage temperature 4°C to 30°C Prepacked HiLoad™ Columns Bed volume HiLoad™ 16/60: 120 ml HiLoad™ 26/60: 320 ml Sample volume HiLoad™ 16/60: < 5 ml HiLoad™ 26/60: < 13 ml Linear flow rate 10-50 cm/h Max. pressure Over the packed bed during operation: 3 bar (42 psi, 0.3 MPa) Column hardware: 5 bar (73 psi, 0.5 MPa) Theoretical plates > 13 000 m-1

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