Jones Lab:Protocols:Kup

Luciferase imaging

To create a sequence file

1) Click on 'Load frames' on Circadian toolbar

2) Choose first image in folder

3) Open into a buffer

4) Doubleclick to open buffer

To annotate image with OAPs

Right click to cancel at any time

1) Click 'Circle OAP'

2) Tap on image to add OAP

3) Resize to suit

4) Click to confirm size

5) Click to confirm position

6) Label

7) Copy using copy command

To extract data

1) Click 'Extract OAP' command on circadian toolbar

Make sure selection = all frames (eg. selection 1-48)

2) Export to Excel