Jones Lab:Protocols:Kup

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(New page: <H3>Luciferase imaging</H3> <H4> To create a sequence file </H4> 1) Click on 'Load frames' on Circadian toolbar 2) Choose first image in folder 3) Open into a buffer 4) Doubleclick to...)
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<H3>Luciferase imaging</H3>
<H3>Luciferase imaging</H3>
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<H4> To capture data </H4>
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1) Focus camera in brightfield mode
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<br>I do this in the dark, with the door open. Remember to set gains high!
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2) Click 'Run TSI' from Circadian toolbar
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3) Change settings as appropriate- time is in seconds
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<br>Remember to save the TRI file, set ND filter to 100% (ie. no filter)
<H4> To create a sequence file </H4>
<H4> To create a sequence file </H4>

Revision as of 06:26, 29 March 2013

Contents

Luciferase imaging

To capture data

1) Focus camera in brightfield mode
I do this in the dark, with the door open. Remember to set gains high!

2) Click 'Run TSI' from Circadian toolbar

3) Change settings as appropriate- time is in seconds
Remember to save the TRI file, set ND filter to 100% (ie. no filter)

To create a sequence file

1) Click on 'Load frames' on Circadian toolbar

2) Choose first image in folder

3) Open into a buffer

4) Doubleclick to open buffer

To annotate image with OAPs

Right click to cancel at any time

1) Click 'Circle OAP'

2) Tap on image to add OAP

3) Resize to suit

4) Click to confirm size

5) Click to confirm position

6) Label

7) Copy using copy command

To extract data

1) Click 'Extract OAP' command on circadian toolbar

Make sure selection = all frames (eg. selection 1-48)

2) Export to Excel

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