Jonesma Lab:Protocols:Chlorophyll: Difference between revisions
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(New page: <H3>Chlorophyll extraction</H3> Modified from Richardson et al. 2002 1) Pre-weigh collection tubes, with grinding beads 2) After tissue collection on N<sub>2</sub>, reweigh collection t...) |
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4) Grind samples on dry ice and spin down | 4) Grind samples on dry ice and spin down | ||
5) Add warmed DMSO and vortex | 5) Add 500μL warmed DMSO and vortex | ||
6) Incubate at 65°C for 30min | 6) Incubate at 65°C for 30min | ||
Revision as of 08:39, 27 February 2014
Chlorophyll extraction
Modified from Richardson et al. 2002
1) Pre-weigh collection tubes, with grinding beads
2) After tissue collection on N2, reweigh collection tubes
3) Pre-warm DMSO to 65°C in glass bottle
4) Grind samples on dry ice and spin down
5) Add 500μL warmed DMSO and vortex
6) Incubate at 65°C for 30min
7) Spin down at 13k for 5min
8) Dilute 1/10 with DMSO
9) Measure at 645nm, 663nm, 750nm
10) Process results as described in Richardson et al.
