Jonesma Lab:Protocols:Chlorophyll

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Current revision (10:47, 22 April 2014) (view source)
 
Line 17: Line 17:
7) Spin down at 13k for 5min
7) Spin down at 13k for 5min
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8) Dilute 1/10 with DMSO
+
8) Dilute 1/10 with DMSO (Final volume 200 μL)
9) Measure at 645nm, 663nm, 750nm
9) Measure at 645nm, 663nm, 750nm
-
10) Process results as described in Richardson et al.
+
10) Process results as described in Richardson et al. - Remember to subtract 750nm from other values

Current revision

Chlorophyll extraction

Modified from Richardson et al. 2002

1) Pre-weigh collection tubes, with grinding beads

2) After tissue collection on N2, reweigh collection tubes

3) Pre-warm DMSO to 65°C in glass bottle

4) Grind samples on dry ice and spin down

5) Add 500μL warmed DMSO and vortex

6) Incubate at 65°C for 30min

7) Spin down at 13k for 5min

8) Dilute 1/10 with DMSO (Final volume 200 μL)

9) Measure at 645nm, 663nm, 750nm

10) Process results as described in Richardson et al. - Remember to subtract 750nm from other values

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