Jonesma Lab:Protocols:ggmod: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: <H3>Golden Gate Ligations</H3> Follow Nicola Patron's startup guide. If there are internal BsaI or BpiI sites add the additional step to the end: 1) Add 1.5 μL T4 ligase buffer and 0.5 ...) |
No edit summary |
||
| Line 1: | Line 1: | ||
<H3>Golden Gate Ligations</H3> | <H3>Golden Gate Ligations</H3> | ||
Follow Nicola Patron's startup guide. If there are internal BsaI or BpiI sites add | Follow Nicola Patron's startup guide. If there are internal BsaI or BpiI sites add these additional steps to the end: | ||
1) Add 1.5 μL T4 ligase buffer and 0.5 μL T4 Ligase | 1) Add 1.5 μL T4 ligase buffer and 0.5 μL T4 Ligase | ||
Latest revision as of 00:40, 22 May 2016
Golden Gate Ligations
Follow Nicola Patron's startup guide. If there are internal BsaI or BpiI sites add these additional steps to the end:
1) Add 1.5 μL T4 ligase buffer and 0.5 μL T4 Ligase
2) Incubate at 16°C for 1 hour
3) Heat to 65°C for 10 min
4) Transform into Top10 cells
