Joyce: Extraction of DNA from an agarose gel: Difference between revisions
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==Procedure== | ==Procedure== |
Revision as of 09:49, 3 April 2012
Procedure
- Remove bands from gel on the preparative setting of a UV illuminator
- Place in an ependorff
- Mash up bands
- Add an equal volume of phenol
- Vortex for 2 minutes
- Place at -70°C for 30 minutes
- Thaw for 10 minutes at 37°C
- Repeat steps 4-7
- Spin at max speed for 1 minute
- Add 20 ul of 3M NaOAc (pH 5.2)
- Spin at full speed for 5 minutes
- Pipette off aqueous phase into a new ependorff
- Adjust salt concentration to 0.3 M
- Extract with 1x phenol, 2x ether, and precipitate