Joyce: Extraction of DNA from an agarose gel: Difference between revisions
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(New page: ==Concept== ******** ==Procedure== #Remove bands from gel on the preparative setting of a UV illuminator #Place in an ependorff #Mash up bands #Add an equal volume of phenol #Vortex for...) |
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#Remove bands from gel on the preparative setting of a UV illuminator | #Remove bands from gel on the preparative setting of a UV illuminator | ||
| Line 12: | Line 9: | ||
#Place at -70°C for 30 minutes | #Place at -70°C for 30 minutes | ||
#Thaw for 10 minutes at 37°C | #Thaw for 10 minutes at 37°C | ||
#Repeat | #Repeat steps 4-7 | ||
#Spin at max speed for 1 minute | #Spin at max speed for 1 minute | ||
#Add 20 ul of 3M NaOAc (pH 5.2) | #Add 20 ul of 3M NaOAc (pH 5.2) | ||
Latest revision as of 09:49, 3 April 2012
- Remove bands from gel on the preparative setting of a UV illuminator
- Place in an ependorff
- Mash up bands
- Add an equal volume of phenol
- Vortex for 2 minutes
- Place at -70°C for 30 minutes
- Thaw for 10 minutes at 37°C
- Repeat steps 4-7
- Spin at max speed for 1 minute
- Add 20 ul of 3M NaOAc (pH 5.2)
- Spin at full speed for 5 minutes
- Pipette off aqueous phase into a new ependorff
- Adjust salt concentration to 0.3 M
- Extract with 1x phenol, 2x ether, and precipitate
