Joyce: Extraction of DNA from an agarose gel

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Revision as of 11:49, 3 April 2012 by Sean E. Lauber (Talk | contribs)
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  1. Remove bands from gel on the preparative setting of a UV illuminator
  2. Place in an ependorff
  3. Mash up bands
  4. Add an equal volume of phenol
  5. Vortex for 2 minutes
  6. Place at -70°C for 30 minutes
  7. Thaw for 10 minutes at 37°C
  8. Repeat steps 4-7
  9. Spin at max speed for 1 minute
  10. Add 20 ul of 3M NaOAc (pH 5.2)
  11. Spin at full speed for 5 minutes
  12. Pipette off aqueous phase into a new ependorff
  13. Adjust salt concentration to 0.3 M
  14. Extract with 1x phenol, 2x ether, and precipitate
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