KAD072606-Construction of pSB1A2-I13450-pACSerZAGGA: Difference between revisions
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[EcoRI][XbaI][tRNA][SpeI][PstI] | [EcoRI][XbaI][tRNA][SpeI][PstI]<br> | ||
Phusion PCR pACSerZAGGA [ca1000/ca1001]<br> | Phusion PCR pACSerZAGGA [ca1000/ca1001]<br> | ||
Digest pACSerZAGGA PCR product [EcoRI/PstI, NEB2, small-97bp]<br> | Digest pACSerZAGGA PCR product [EcoRI/PstI, NEB2, small-97bp]<br> | ||
Paste into pSB1A3-I13450 [MfeI/NsiI, NEB2, large-2165bp]<br> | Paste into pSB1A3-I13450 [MfeI/NsiI, NEB2, large-2165bp]<br> | ||
Product is pSB1A3-J23037<br> | Product is pSB1A3-J23037<br> | ||
Latest revision as of 11:35, 7 August 2006
[EcoRI][XbaI][tRNA][SpeI][PstI]
Phusion PCR pACSerZAGGA [ca1000/ca1001]
Digest pACSerZAGGA PCR product [EcoRI/PstI, NEB2, small-97bp]
Paste into pSB1A3-I13450 [MfeI/NsiI, NEB2, large-2165bp]
Product is pSB1A3-J23037
