KAD072606-Construction of pSB1A2-I13450-pACSerZAGGA

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[EcoRI][XbaI][tRNA][SpeI][PstI]
Phusion PCR pACSerZAGGA [ca1000/ca1001]
Digest pACSerZAGGA PCR product [EcoRI/PstI, NEB2, small-97bp]
Paste into pSB1A3-I13450 [MfeI/NsiI, NEB2, large-2165bp]
Product is pSB1A3-J23037

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