Kafatos:10x PBS

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{{Kafatos/Christophides Lab}}
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=== 10x PBS ===
=== 10x PBS ===
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'''Add H2O to 1L'''
'''Add H2O to 1L'''
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'''Autoclave for 20 minutes on liquid cycle. Store at RT. '''<BR>
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'''Autoclave on liquids cycle. Store at RT.'''<BR>
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[[Category:Material]] [[Category:Buffer]]

Revision as of 09:16, 5 May 2009

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10x PBS

Combine the following:

80 g NaCl
2 g KCl
14.4 g Na2HPO4
2.4 g KH2PO4
800 mL distilled H2O


Adjust pH to 7.4 with HCl
Add H2O to 1L
Autoclave on liquids cycle. Store at RT.

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