Kafatos:10x PBS: Difference between revisions
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{{Kafatos/Christophides Lab}} | |||
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=== 10x PBS === | === 10x PBS === | ||
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'''Add H2O to 1L''' | '''Add H2O to 1L''' | ||
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'''Autoclave | '''Autoclave on liquids cycle. Store at RT.'''<BR> | ||
[[Category:Material]] [[Category:Buffer]] | |||
Revision as of 06:16, 5 May 2009
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10x PBS
Combine the following:
- 80 g NaCl
- 2 g KCl
- 14.4 g Na2HPO4
- 2.4 g KH2PO4
- 800 mL distilled H2O
Adjust pH to 7.4 with HCl
Add H2O to 1L
Autoclave on liquids cycle. Store at RT.
