Kafatos:4x SDS PAGE Reducing Sample Buffer: Difference between revisions
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== 4x SDS PAGE Reducing Sample Buffer == | == 4x SDS PAGE Reducing Sample Buffer == | ||
Revision as of 15:13, 2 March 2006
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4x SDS PAGE Reducing Sample Buffer
25mL Milli-Q Water
25mL 1M Tris, pH 6.8
40mL Glycerol
8g SDS
8mL ß-Mercapto-ethanol
400mg Bromophenol Blue
Once SDS and BB are dissolved, filter with a 0.2µm syringe filter.
Store aliquots in -20ºC
