Kafatos:4x SDS PAGE Reducing Sample Buffer

From OpenWetWare

Revision as of 14:19, 9 March 2006 by Smeister (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Home Lab Members Research Publications Contact

4x SDS PAGE Reducing Sample Buffer

25mL Milli-Q Water
25mL 1M Tris, pH 6.8
40mL Glycerol
8g SDS
8mL ß-Mercapto-ethanol
400mg Bromophenol Blue

Once SDS and BB are dissolved, filter with a 0.2µm syringe filter.
Store aliquots in -20ºC

Personal tools