Kafatos:Making 1kb+ Ladder Stock: Difference between revisions
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Mpovelones (talk | contribs) (New page: {{Kafatos/Christophides Lab}} ==Overview== Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots ==Materials== 250uL 1kb+ DNA Ladder [1ug/uL...) |
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==Materials== | ==Materials== | ||
250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen) | 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)<BR> | ||
500uL 5x DNA Loading Buffer | 500uL 5x DNA Loading Buffer<BR> | ||
1750uL AccuGene Water (Lonza) | 1750uL AccuGene Water (Lonza)<BR> | ||
==Procedure== | ==Procedure== | ||
Revision as of 09:18, 5 November 2009
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Overview
Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots
Materials
250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
500uL 5x DNA Loading Buffer
1750uL AccuGene Water (Lonza)
Procedure
- Add the 3 components above together
- Mix well and make 5 x 500uL aliquots
- Leave one working stock at 4C and keep the other frozen at -20C
- Use 10uL of this stock per lane (1ug of ladder per lane)
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
