Kafatos:Making 1kb+ Ladder Stock

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(New page: {{Kafatos/Christophides Lab}} ==Overview== Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots ==Materials== 250uL 1kb+ DNA Ladder [1ug/uL...)
Current revision (12:18, 5 November 2009) (view source)
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==Materials==
==Materials==
-
250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
+
#250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
-
500uL 5x DNA Loading Buffer
+
#500uL 5x DNA Loading Buffer
-
1750uL AccuGene Water (Lonza)
+
#1750uL AccuGene Water (Lonza)
==Procedure==
==Procedure==

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Contents

Overview

Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots

Materials

  1. 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
  2. 500uL 5x DNA Loading Buffer
  3. 1750uL AccuGene Water (Lonza)

Procedure

  1. Add the 3 components above together
  2. Mix well and make 5 x 500uL aliquots
  3. Leave one working stock at 4C and keep the other frozen at -20C
  4. Use 10uL of this stock per lane (1ug of ladder per lane)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

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