Kafatos:Making 1kb+ Ladder Stock - Revision history http://www.openwetware.org/index.php?title=Kafatos:Making_1kb%2B_Ladder_Stock&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Wed, 22 May 2013 16:05:54 GMT Mpovelones: /* Materials */ http://www.openwetware.org/index.php?title=Kafatos:Making_1kb%2B_Ladder_Stock&diff=364464&oldid=prev <p><span class="autocomment">Materials</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 16:18, 5 November 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)<del class="diffchange diffchange-inline">&lt;BR&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>500uL 5x DNA Loading Buffer<del class="diffchange diffchange-inline">&lt;BR&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>500uL 5x DNA Loading Buffer</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1750uL AccuGene Water (Lonza)<del class="diffchange diffchange-inline">&lt;BR&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>1750uL AccuGene Water (Lonza)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td></tr> <!-- diff generator: internal 2013-05-22 16:05:54 --> </table> Thu, 05 Nov 2009 16:18:30 GMT Mpovelones http://www.openwetware.org/wiki/Talk:Kafatos:Making_1kb%2B_Ladder_Stock Mpovelones: /* Materials */ http://www.openwetware.org/index.php?title=Kafatos:Making_1kb%2B_Ladder_Stock&diff=364463&oldid=prev <p><span class="autocomment">Materials</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 16:18, 5 November 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)<ins class="diffchange diffchange-inline">&lt;BR&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>500uL 5x DNA Loading Buffer</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>500uL 5x DNA Loading Buffer<ins class="diffchange diffchange-inline">&lt;BR&gt;</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1750uL AccuGene Water (Lonza)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1750uL AccuGene Water (Lonza)<ins class="diffchange diffchange-inline">&lt;BR&gt;</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Procedure==</div></td></tr> <!-- diff generator: internal 2013-05-22 16:05:54 --> </table> Thu, 05 Nov 2009 16:18:06 GMT Mpovelones http://www.openwetware.org/wiki/Talk:Kafatos:Making_1kb%2B_Ladder_Stock Mpovelones: New page: {{Kafatos/Christophides Lab}} ==Overview== Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots ==Materials== 250uL 1kb+ DNA Ladder [1ug/uL... http://www.openwetware.org/index.php?title=Kafatos:Making_1kb%2B_Ladder_Stock&diff=364462&oldid=prev <p>New page: {{Kafatos/Christophides Lab}} ==Overview== Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots ==Materials== 250uL 1kb+ DNA Ladder [1ug/uL...</p> <p><b>New page</b></p><div>{{Kafatos/Christophides Lab}}<br /> <br /> ==Overview==<br /> <br /> Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots<br /> <br /> ==Materials==<br /> 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)<br /> 500uL 5x DNA Loading Buffer<br /> 1750uL AccuGene Water (Lonza)<br /> <br /> ==Procedure==<br /> #Add the 3 components above together<br /> #Mix well and make 5 x 500uL aliquots<br /> #Leave one working stock at 4C and keep the other frozen at -20C<br /> #Use 10uL of this stock per lane (1ug of ladder per lane)<br /> <br /> ==Notes==<br /> Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!<br /> #List troubleshooting tips here. <br /> #You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.<br /> #Anecdotal observations that might be of use to others can also be posted here. <br /> <br /> Please sign your name to your note by adding &lt;font face=&quot;courier&quot;&gt;&lt;nowiki&gt;'''*~~~~''':&lt;/nowiki&gt;&lt;/font&gt; to the beginning of your tip.<br /> <br /> <br /> <br /> ==Contact==<br /> *Who has experience with this protocol?<br /> <br /> or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. <br /> <br /> &lt;!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --&gt;<br /> [[Category:DNA]]<br /> [[Category:Protocol]]<br /> <br /> &lt;!-- Move the relevant categories above this line to tag your protocol with the label<br /> <br /> [[Category:Protocol]]<br /> [[Category:Needs attention]] <br /> [[Category:In vitro]]<br /> <br /> [[Category:In vivo]]<br /> <br /> [[Category:In silico]]<br /> <br /> [[Category:DNA]]<br /> <br /> [[Category:RNA]]<br /> <br /> [[Category:Protein]]<br /> <br /> [[Category:Chemical]]<br /> <br /> [[Category:Escherichia coli]]<br /> <br /> [[Category:Yeast]]<br /> --&gt;</div> Thu, 05 Nov 2009 16:17:34 GMT Mpovelones http://www.openwetware.org/wiki/Talk:Kafatos:Making_1kb%2B_Ladder_Stock