Kafatos:Ordering: Difference between revisions
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==Primers== | ==Primers== | ||
We order primers from [http://www.sigmaaldrich.com/Brands/Sigma_Genosys.html/ Sigma-Genosys]<BR> | We order primers from [http://www.sigmaaldrich.com/Brands/Sigma_Genosys.html/ Sigma-Genosys]<BR> | ||
Orders require | Orders require | ||
* Name (35 characters fit on the tube label) | * <bold>Name</bold> (35 characters fit on the tube label) | ||
* Sequence | * <bold>Sequence</bold> [[Media:Sigma_oligo_web_ordering.pdf | PDF with detailed instructions and modification codes]] | ||
* Scale | * <bold>Scale</bold> | ||
** 0.025, 0.05, 0.2, 1, 10 and 15 µmol | ** 0.025, 0.05, 0.2, 1, 10 and 15 µmol | ||
** 0.025 must be between 12 and 35 bases and can only be desalted | ** 0.025 must be between 12 and 35 bases and can only be desalted | ||
* Purification | * <bold>Purification</bold> | ||
**DST (desalted), RP1 (reverse phase cartridge), HPLC and PAGE | ** DST (desalted), RP1 (reverse phase cartridge), HPLC and PAGE | ||
[[Media:Oligo_order_form.xls | Excel template for primer orders]] Email as an attachment to [[Kafatos:Povelones%2C_Michael | Pove]] or [[Kafatos:Osta%2C_Mike | Mike]]<BR> | [[Media:Oligo_order_form.xls | Excel template for primer orders]] Email as an attachment to [[Kafatos:Povelones%2C_Michael | Pove]] or [[Kafatos:Osta%2C_Mike | Mike]]<BR> | ||
<BR> | |||
==DNA Sequencing== | ==DNA Sequencing== | ||
<font color="red">...in progress...</FONT> | <font color="red">...in progress...</FONT> | ||
Revision as of 05:37, 8 August 2006
ICIS online ordering system (requires password)
Primers
We order primers from Sigma-Genosys
Orders require
- <bold>Name</bold> (35 characters fit on the tube label)
- <bold>Sequence</bold> PDF with detailed instructions and modification codes
- <bold>Scale</bold>
- 0.025, 0.05, 0.2, 1, 10 and 15 µmol
- 0.025 must be between 12 and 35 bases and can only be desalted
- <bold>Purification</bold>
- DST (desalted), RP1 (reverse phase cartridge), HPLC and PAGE
Excel template for primer orders Email as an attachment to Pove or Mike
DNA Sequencing
...in progress...
