Kafatos:Ordering: Difference between revisions

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==Ordering Information==
==Ordering Information==


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===For Lab Orderers===
===For Lab Orderers===
[http://www.icis.ic.ac.uk/ ICIS online ordering system (requires password)]
[http://www.icis.ic.ac.uk/ ICIS online ordering system (requires password)]
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===Primers===
===Primers===
We order primers from [http://www.sigmaaldrich.com/Brands/Sigma_Genosys.html/ Sigma-Genosys]<BR>
We order primers from [http://www.sigmaaldrich.com/Brands/Sigma_Genosys.html/ Sigma-Genosys]<BR>
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* <b>Purification</b>
* <b>Purification</b>
** DST (desalted), RP1 (reverse phase cartridge), HPLC and PAGE
** DST (desalted), RP1 (reverse phase cartridge), HPLC and PAGE
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===DNA Sequencing===
===DNA Sequencing===
We have sequencing done by The Natural History Museum<BR>
We have sequencing done by The Natural History Museum<BR>
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** Small PCR products: 10 &mu;l of Qiagen PCR purified DNA at 100 ng/&mu;l is sufficient.
** Small PCR products: 10 &mu;l of Qiagen PCR purified DNA at 100 ng/&mu;l is sufficient.
** Other DNA? Please update with information if you enquire about other templates.
** Other DNA? Please update with information if you enquire about other templates.
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Revision as of 11:53, 8 August 2006

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Ordering Information

For Lab Orderers

ICIS online ordering system (requires password)


Primers

We order primers from Sigma-Genosys
Please use this Excel template and email as an attachment to Pove or Mike

Orders require the following information:

  • Name (35 characters fit on the tube label)
  • Sequence PDF with detailed instructions and modification codes
  • Scale
    • 0.025, 0.05, 0.2, 1, 10 and 15 μmol
    • 0.025 must be between 12 and 35 bases and can only be desalted
  • Purification
    • DST (desalted), RP1 (reverse phase cartridge), HPLC and PAGE


DNA Sequencing

We have sequencing done by The Natural History Museum
Contact: Juiliad Llewellyn-Hughes for info on sample requirements and to schedule a drop-off time.
Tel: 0207 942 5352
Tel: 0207 942 5347

  • Get a PO Number from Pove or Mike. The cost is £8/reaction.
  • Complete this form and make a copy.
  • Contact Juilad to schedule a drop-off time
  • Primers
    • Common primers: T3, T7 etc are supplied by the facility.
    • Custom primers: Submit 10 μl of a 10 μM stock to the facility.
  • Templates
    • Small PCR products: 10 μl of Qiagen PCR purified DNA at 100 ng/μl is sufficient.
    • Other DNA? Please update with information if you enquire about other templates.