Kanamycin: Difference between revisions
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==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The ''Kan<sup>R</sup>'' gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as [[neomycin]]. | Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The ''Kan<sup>R</sup>'' gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as [[neomycin]] or [[gentamycin]]. | ||
==Working Concentration and Stock Solution== | ==Working Concentration and Stock Solution== | ||
Working concentration is | Working concentration is 50 μg/ml, or 25 μg/ml for low-copy plasmids like [http://openwetware.org/wiki/Bacterial_artificial_chromosomes#PAC_vectors PACs]. Stock solution is 35 mg/ml in water (kanamycin is insoluble in 50% alcohol). | ||
==References== | ==References== | ||
Molecular Cloning: A Laboratory Manual, Vol 1. | Molecular Cloning: A Laboratory Manual, Vol 1. | ||
==Links== | |||
[http://bacpac.chori.org/clone-handling.htm Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources] | |||
[[Category:Material]] [[Category:Antibiotic]] | |||
Latest revision as of 18:52, 12 April 2010
Mode of Action
Bacteriocidal. Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.
Mechanism of Resistance
Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The KanR gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as neomycin or gentamycin.
Working Concentration and Stock Solution
Working concentration is 50 μg/ml, or 25 μg/ml for low-copy plasmids like PACs. Stock solution is 35 mg/ml in water (kanamycin is insoluble in 50% alcohol).
References
Molecular Cloning: A Laboratory Manual, Vol 1.
Links
Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources
