Kansai Team's Project

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・Abstract

DNA Origami is a useful technique to construct various nano structures and devices.In our project, we decided to apply DNA Origami method to represent art .We play nano-sized Flip-Book using DNA Origami nano structures.We adopted Aesop's fables as the subject.Three major components, The Hare,The Tortoise, and The Ground, are formed within one DNA Origami molecule.To show the whole story with AFM images, we will have to construct many patterns. But it will require unrealistic cost. We solved this problem by connecting each components through a long linker and combining each of them at proper positions.

・ Flip-Book

In this project, we show you Nano Flip-Book by using AFM images. In other words, we make a kind of nanoscale animation. In Japan, we often draw pictures on a chip of note or books. We flip through them quickly and it makes pictures look moving because of afterimages.

・Aesop’s Fables

The fairy tail "The Tortoise and the Hare" in this Aesop’s Fables is very famous around the world. We designed DNA origami in the shape of “The Tortoise”, “The Hare” and “The Ground” and succeeded to observe AFM images of them. We play “Nano-Flip-Book" by flipping through them quickly.

・DNA origami

This DNA origami used about 200 staples DNA and consisted of major three components; “The Tortoise, the Hare and the Ground”, connected by flexible linker at the both ends. if we make every frame of the stay with DNA origami, we will need thousands of staples. This will surely require astronomic costs. So we solved this problem by using proper joint staples to join DNA origami “The Ground and each character”. For example, when we moved DNA origami from “ The Tortoise” to “the Hare”, we used linker on end of the Ground and made linker between “The Tortoise and the Hare” because if we don’t make linker, we will need new many staples. We observed DNA origami “The Tortoise and the Hare” by using AFM many times. In this way, without using thousand of staples DNA, we succeeded in operating DNA origami “Tortoise” and “Hare” by using hundreds of staples to play Nano- “The Tortoise and the Hare” and successfully saved the staples cost.

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Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the ground”