Kansai Team's Protocols: Difference between revisions
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'''Protocols'''<br> | |||
・Materials | |||
Staples DNA were purchased from IDT ( America ) | |||
'''A. Self assembly of DNA origami'''<br> | |||
The formation of DNA origami was performed with M13mp18 ssDNA (4 nM, Takara, Japan), staples DNA (20 nM for each strand) in a solution containing Tris (40 mM), acetic acid (20 mM), EDTA (10 mM), and magnesium acetate (12.5 mM, 1 X TAE/Mg buffer, 50 µL). This mixture was cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands. | |||
'''B. AFM observation''' | |||
AFM imaging of DNA origami was performed on a E-sweep system (SII, Japan). The mixture (1 µL) was deposited on freshly cleaved mica, additional 1X TAE/Mg buffer (40 µL) was added, and the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan). | |||
Revision as of 21:58, 29 August 2013
Protocols
| Top | Team | Project | Results | Protocols |
Protocols
・Materials
Staples DNA were purchased from IDT ( America )
A. Self assembly of DNA origami
The formation of DNA origami was performed with M13mp18 ssDNA (4 nM, Takara, Japan), staples DNA (20 nM for each strand) in a solution containing Tris (40 mM), acetic acid (20 mM), EDTA (10 mM), and magnesium acetate (12.5 mM, 1 X TAE/Mg buffer, 50 µL). This mixture was cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands.
B. AFM observation
AFM imaging of DNA origami was performed on a E-sweep system (SII, Japan). The mixture (1 µL) was deposited on freshly cleaved mica, additional 1X TAE/Mg buffer (40 µL) was added, and the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan).
