Kansai Team's Results: Difference between revisions
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<td width="180" bgcolor="#00cc66">[[Kansai Team's Protocols|<font face="Comic Sans MS,cursive,Arial" color="white">Protocols</font>]] </td> | <td width="180" bgcolor="#00cc66">[[Kansai Team's Protocols|<font face="Comic Sans MS,cursive,Arial" color="white">Protocols</font>]] </td> | ||
<td width="180" bgcolor="#00cc66">[[Kansai Team's Sources|<font face="Comic Sans MS,cursive,Arial" color="white">Sources</font>]] </td> | <td width="180" bgcolor="#00cc66">[[Kansai Team's Sources|<font face="Comic Sans MS,cursive,Arial" color="white">Sources</font>]] </td> | ||
<td width="180" bgcolor="#00cc66">[[Kansai Team's Etc.|<font face="Comic Sans MS,cursive,Arial" color="white">Etc.</font>]] </td> | |||
</tr> | </tr> | ||
</table> | </table> | ||
<div align="left">[[image:DNA_Usagi_to_Kame_亀兎両端リンカー2.jpg|600px]]</div> | <div align="left">[[image:DNA_Usagi_to_Kame_亀兎両端リンカー2.jpg|600px]]</div> | ||
Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” We designed this whole structure by hands, without using caDNAno at all. | Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” This structure is called T1H6, which denotes the Tortoise at position 1 and the Hare at position 6. We designed this whole structure by hands, without using caDNAno at all. | ||
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[[image: | <div align="left">[[image:AFM_of_usakame.png|600px]]</div> | ||
Fig. 2. AFM image of DNA origami “The Tortoise, the Hare and the | Fig. 2. AFM image of DNA origami “The Tortoise, the Hare and the ground (T1H6)” | ||
<div align="left">[[image: | <div align="left">[[image:UsakameAFM.png|600px]]</div> | ||
Fig. 3. | Fig. 3. A zoom-up image of DNA origami “The Tortoise, the Hare and the ground (T1H6)” | ||
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<div align="left">[[image:Usakameto.png|600px]]</div> | <div align="left">[[image:Usakameto.png|600px]]</div> | ||
Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the | Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the ground (T1H6)” | ||
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DNA origami “The Tortoise and the Hare (T1H6)” was clearly of observed by using AFM (Fig. 2.) The Tortoise is located on the left side and the Hare is located on the right of the ground. But in the AFM image, we also observed two motifs joined to each other. We solved this problem by adding T4 loops to the staples. But this was not enough. We assumed that single-stranded Loops are also responsible for aggregation. So we added short strands which are partially complementarity to the Loops (Fig. 1.) | |||
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<div align="left"> | <div align="left"> | ||
[[image:亀端兎1_.jpg|300px]][[image:亀端兎2_.jpg|300px]][[image:亀端兎3_.jpg|300px]] | [[image:亀端兎1_.jpg|300px]][[image:亀端兎2_.jpg|300px]][[image:亀端兎3_.jpg|300px]][[image:亀端兎4_.jpg|300px]][[image:亀端兎5_.jpg|300px]]</div> | ||
[[image:亀端兎4_.jpg|300px]][[image:亀端兎5_.jpg|300px]] | <div align="left">Fig 5. (a) Other DNA origami designs for different scenses.</div><br><br> | ||
[[image:U_to_K_5.jpg|300px]][[image:U_to_K_4.jpg|300px]][[image:U_to_K_3.jpg|300px]][[image:U_to_K_2.jpg|300px]][[image:U_to_K_1.jpg|300px]]</div> | <div align="left">[[image:U_to_K_5.jpg|300px]][[image:U_to_K_4.jpg|300px]][[image:U_to_K_3.jpg|300px]][[image:U_to_K_2.jpg|300px]][[image:U_to_K_1.jpg|300px]]</div><br><br> | ||
<div align="left">Fig 5. (a-1) Other AFM images of DNA origami for different scenses.</div><br><br> | |||
<div align="left">[[Image:Old UtoK LK Khashi U1.003.jpg|500px]]<br>[[Image:Old UtoK LK Khashi U2.003.jpg|500px]]<br>[[Image:Old UtoK LK Khashi U3.000.jpg|500px]]<br>[[Image:Old UtoK LK Khashi U4.000.jpg|500px]]<br>[[Image:Old UtoK LK Khashi U5.002.bmp|500px]]<br></div> | |||
We could observe another five frames. | |||
[[image:亀2兎端_.jpg|300px]][[image:亀3兎端_.jpg|300px]][[image:亀4兎端_.jpg|300px]][[image:亀5兎端_.jpg|300px]] | <div align="left"> | ||
[[Image:K1Uhashi.png|300px]][[image:亀2兎端_.jpg|300px]][[image:亀3兎端_.jpg|300px]][[image:亀4兎端_.jpg|300px]][[image:亀5兎端_.jpg|300px]] | |||
</div><br> | </div><br> | ||
Fig 5. Other DNA origami designs for different scenses | Fig 5. (b) Other DNA origami designs for different scenses. | ||
<br><br> | |||
<div align="left">[[Image:U to K U hashi K 1.png|300px]][[Image:U to K U hashi K 2.png|300px]][[Image:U to K U hashi K 3.png|300px]][[Image:U to K U hashi K 4.png|300px]][[Image:U to K U hashi K 5.png|300px]]<br><br></div> | |||
<div align="left">Fig 5. (b-1) Other AFM images of DNA origami for different scenses.</div><br><br> | |||
<div align="left"> | |||
[[Image:Utok2.000.bmp|500px]]<br>[[Image:Utok3.000.bmp|500px]]<br>[[Image:Utok3.001.bmp|500px]]<br>[[Image:Utok4.003.bmp|500px]]<br>[[Image:Utok3.002.bmp|500px]]<br></div><br> | |||
We could observe another five frames. | |||
<div align="left"> | |||
[[Image:UtoK AFM.gif|800px]]</div> | |||
We connected AFM images we observed.<br> | |||
・We also designed animated background with "Trees."<br> | |||
<div align="left">[[Image:Trees.png]]</div> | |||
Fig. 6. Designs of DNA origami ”Trees."<br> | |||
Latest revision as of 05:41, 26 October 2013
Results
| Top | Team | Project | Results | Protocols | Sources | Etc. |
Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” This structure is called T1H6, which denotes the Tortoise at position 1 and the Hare at position 6. We designed this whole structure by hands, without using caDNAno at all.
Fig. 2. AFM image of DNA origami “The Tortoise, the Hare and the ground (T1H6)”
Fig. 3. A zoom-up image of DNA origami “The Tortoise, the Hare and the ground (T1H6)”
Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the ground (T1H6)”
DNA origami “The Tortoise and the Hare (T1H6)” was clearly of observed by using AFM (Fig. 2.) The Tortoise is located on the left side and the Hare is located on the right of the ground. But in the AFM image, we also observed two motifs joined to each other. We solved this problem by adding T4 loops to the staples. But this was not enough. We assumed that single-stranded Loops are also responsible for aggregation. So we added short strands which are partially complementarity to the Loops (Fig. 1.)
Fig 5. (a) Other DNA origami designs for different scenses.
Fig 5. (a-1) Other AFM images of DNA origami for different scenses.
We could observe another five frames.
Fig 5. (b) Other DNA origami designs for different scenses.
Fig 5. (b-1) Other AFM images of DNA origami for different scenses.
We could observe another five frames.
We connected AFM images we observed.
・We also designed animated background with "Trees."
Fig. 6. Designs of DNA origami ”Trees."
