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Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” We designed this whole structure by hands, without using caDNAno at all.


Fig. 2. AFM image of DNA origami “The Tortoise, the Hare and the ground”

Fig. 3. AFM image of DNA origami “The Tortoise, the Hare and the ground”

Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the ground”

We succeeded to observe DNA origami “The Tortoise and the Hare” by using AFM. (Fig. 2.) The Tortoise is located on the left side and the Hare is located on the right of the ground. But in the AFM image, we also observed two motifs joined to each other (Fig. 3.) We solved this problem by adding free Ts to the staples and staples with complementary linkers because of preventing linkers from stacking.(Fig. 1.)

Fig 5. Other DNA origami designs for different scenses