Kansai Team's Results: Difference between revisions
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<div align="left">[[image:DNA_Usagi_to_Kame_亀兎両端リンカー2.jpg|600px]]</div> | <div align="left">[[image:DNA_Usagi_to_Kame_亀兎両端リンカー2.jpg|600px]]</div> | ||
Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” We designed this whole structure by hands, without using caDNAno at all. | Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” This structure is called T1H6, which denotes the Tortoise at position 1 and the Hare at position 6. We designed this whole structure by hands, without using caDNAno at all. | ||
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<div align="left">[[image:AFM_of_usakame.png|600px]]</div> | |||
Fig. 2. AFM image of DNA origami “The Tortoise, the Hare and the ground (T1H6)” | |||
[[image:UsakameAFM.png|600px]]<br> | [[image:UsakameAFM.png|600px]]<br> | ||
Fig. 3. A zoom-up image of DNA origami “The Tortoise, the Hare and the ground (T1H6)” | |||
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<div align="left">[[image:Usakameto.png|600px]]</div> | <div align="left">[[image:Usakameto.png|600px]]</div> | ||
Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the | Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the ground (T1H6)” | ||
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DNA origami “The Tortoise and the Hare (T1H6)” was clearly of observed by using AFM (Fig. 2.) The Tortoise is located on the left side and the Hare is located on the right of the ground. But in the AFM image, we also observed two motifs joined to each other. We solved this problem by adding T4 loops to the staples. But this was not enough. We assumed that single-stranded Loops are also responsible for aggregation. So we added short strands which are partially complementarity to the Loops (Fig. 1.) We will make the Trees with staples on the Ground. If we observed AFM images "the Trees", we will connect Two AFM images "The Tortoise, the Hare and the Ground” and "the Trees". (Fig. 6.) | |||
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Fig 5. Other DNA origami designs for different scenses | Fig 5. Other DNA origami designs for different scenses | ||
<div align="left">[[Image:Trees.png]] | <div align="left">[[Image:Trees.png]] | ||
Fig. 6. | Fig. 6. Designs of DNA origami ”Trees" | ||
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Revision as of 03:37, 24 October 2013
Results
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Fig. 1. The design of DNA origami “The Tortoise, the Hare and the ground.” This structure is called T1H6, which denotes the Tortoise at position 1 and the Hare at position 6. We designed this whole structure by hands, without using caDNAno at all.
Fig. 2. AFM image of DNA origami “The Tortoise, the Hare and the ground (T1H6)”
Fig. 3. A zoom-up image of DNA origami “The Tortoise, the Hare and the ground (T1H6)”
Fig. 4. Illustration of DNA origami “The Tortoise, the Hare and the ground (T1H6)”
DNA origami “The Tortoise and the Hare (T1H6)” was clearly of observed by using AFM (Fig. 2.) The Tortoise is located on the left side and the Hare is located on the right of the ground. But in the AFM image, we also observed two motifs joined to each other. We solved this problem by adding T4 loops to the staples. But this was not enough. We assumed that single-stranded Loops are also responsible for aggregation. So we added short strands which are partially complementarity to the Loops (Fig. 1.) We will make the Trees with staples on the Ground. If we observed AFM images "the Trees", we will connect Two AFM images "The Tortoise, the Hare and the Ground” and "the Trees". (Fig. 6.)
We could observe five AFM images on whichThe Hare moves to the left
Fig 5. Other DNA origami designs for different scenses
Fig. 6. Designs of DNA origami ”Trees"
