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*fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy) | *fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy) | ||
==Publications== | ==Publications== | ||
Revision as of 10:51, 12 September 2006
Education/Work Experience
- Ph.D. in Chemistry, The Scripps Research Institute, 2001 (with Gerald Joyce)
- B.S. in Chemistry, University of Maryland College Park, 1996
- B.S. in Psychology, University of Maryland College Park, 1996
Science stuff I know something about...
- tmRNA (or SsrA/10Sa RNA for you purists)
- translation
- energy-dependent proteolysis in bacteria
- The RNA World
- molecular biology in a world without kits
- in vitro selection and evolution of nucleic acids
- generation of nucleic acid and protein libraries
- in vitro RNA techniques
- nucleic acid-protein interactions (in vitro analysis and bacterial in vivo analysis)
- fishing experiments to look for things that interact with your RNA/DNA/protein of interest
- PCR (even colony PCR, although it is not a separate skill)
- bacterial protein expression/purification
- in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
- molecular cloning
- Western/Northern/Southern blot analysis
- oligonucleotide synthesis
- RNA/DNA sequencing ("old school" and "new school" techniques)
- chemical and enzymatic structural probing of RNA/DNA
- gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
- enzyme kinetics
- radiolabeling nucleic acids and proteins
- familiarity with some biophysical techniques (a bit of CD spectroscopy, a small amount of analytical ultracentrifugation)
- fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy)
Publications
Engineering controllable protein degradation.
McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Mol. Cell, 22, 701-707.
McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.
A Glimpse into tmRNA-mediated Ribosome Rescue.
Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003) Science 300, 127–130. (comment)
In Search of an RNA Replicase Ribozyme.
McGinness, K.E. & Joyce, G.F. (2003) Chem. Biol. 10, 5–14. (review)
McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002) Chem. Biol. 9, 585–596.
RNA-Catalyzed RNA Ligation on an External RNA Template.
McGinness, K.E. & Joyce, G.F. (2002) Chem. Biol. 9, 297–307.
Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.
McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991.
Contact
If you're on OWW, post a message on my talk page, or send me an email.
If you're not on OWW, join now! In the meantime, you can email me. Just take my user name, hey that's kathmc, then add on mit, a period, and edu.
Send me stuff!
31 Ames St.
68-589
Cambridge, MA 02139
Fun facts
- Who's visiting? (Thanks Barry!)
<html> <a href="http://clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/Kathleen_McGinness" id="clustrMapsLink"><img src="http://clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/Kathleen_McGinness" border=1 alt="Locations of visitors to this page"onError="this.onError=null; this.src='http://www.meetomatic.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com/'"> </a> </html>
- Other former WFJL crew on OWW: Roshan Kumar, William Shih
Important things I've learned through real life experiences.
- If you can't fix it with duct tape, a safety pin, or both, you should probably just throw it out.
- Almost everything is better with bacon.
- Never underestimate the importance of your karass (although I like this definition better) and beware of granfalloons.
