Kathleen McGinness: Difference between revisions
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==Publications== | ==Publications== | ||
'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17317664&query_hl=1&itool=pubmed_docsum Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery.]''' | |||
McGinness K.E., Bolon, D.N., Kaganovich, M., Baker, T.A. & Sauer, R.T. (2007) J. Biol. Chem., Feb 22 [Epub ahead of print] | |||
'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16762842&query_hl=1&itool=pubmed_docsum Engineering controllable protein degradation.]''' | '''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16762842&query_hl=1&itool=pubmed_docsum Engineering controllable protein degradation.]''' | ||
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'''[http://www.jbc.org/cgi/content/full/277/4/2987 Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.]''' | '''[http://www.jbc.org/cgi/content/full/277/4/2987 Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.]''' | ||
McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991. | McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991. | ||
==Contact== | ==Contact== | ||
Revision as of 09:08, 25 February 2007
Work Experience
- Scientist, Archemix Corp. (2006–??)
- Postdoc in the Sauer lab at MIT (2001–2006)
Education
- Ph.D. in Chemistry, The Scripps Research Institute, 2001 (with Gerald Joyce)
- B.S. in Chemistry, University of Maryland College Park, 1996 (with Sarah Woodson)
- B.S. in Psychology, University of Maryland College Park, 1996
Science stuff I know something about...
- tmRNA (or SsrA/10Sa RNA for you purists)
- translation
- energy-dependent proteolysis in bacteria
- The RNA World
- molecular biology in a world without kits
- in vitro selection and evolution of nucleic acids
- generation of nucleic acid and protein libraries
- in vitro RNA techniques
- nucleic acid-protein interactions (in vitro analysis and bacterial in vivo analysis)
- fishing experiments to look for things that interact with your RNA/DNA/protein of interest
- PCR (even colony PCR, although it is not a separate skill)
- bacterial protein expression/purification
- in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
- molecular cloning
- Western/Northern/Southern blot analysis
- oligonucleotide synthesis
- RNA/DNA sequencing ("old school" and "new school" techniques)
- chemical and enzymatic structural probing of RNA/DNA
- gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
- enzyme kinetics
- radiolabeling nucleic acids and proteins
- familiarity with some biophysical techniques (a bit of CD spectroscopy, a small amount of analytical ultracentrifugation)
- fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy)
Publications
Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery.
McGinness K.E., Bolon, D.N., Kaganovich, M., Baker, T.A. & Sauer, R.T. (2007) J. Biol. Chem., Feb 22 [Epub ahead of print]
Engineering controllable protein degradation.
McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Mol. Cell, 22, 701-707.
McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.
A Glimpse into tmRNA-mediated Ribosome Rescue.
Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003) Science 300, 127–130. (comment)
In Search of an RNA Replicase Ribozyme.
McGinness, K.E. & Joyce, G.F. (2003) Chem. Biol. 10, 5–14. (review)
McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002) Chem. Biol. 9, 585–596.
RNA-Catalyzed RNA Ligation on an External RNA Template.
McGinness, K.E. & Joyce, G.F. (2002) Chem. Biol. 9, 297–307.
Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.
McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991.
Contact
If you're on OWW, post a message on my talk page, or send me an email.
If you're not on OWW, join now!
Fun facts
- Who's visiting? (Thanks Barry!)
<html> <a href="http://clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/Kathleen_McGinness" id="clustrMapsLink"><img src="http://clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/Kathleen_McGinness" border=1 alt="Locations of visitors to this page"onError="this.onError=null; this.src='http://www.meetomatic.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com/'"> </a> </html>
- Other former WFJL crew on OWW: Roshan Kumar, William Shih
- Related former TSRI folk on OWW: Plachikkat Radha
Important things I've learned through real life experiences.
- If you can't fix it with duct tape, a safety pin, or both, you should probably just throw it out.
- Almost everything is better with bacon.
- Never underestimate the importance of your karass (although I like this definition better) and beware of granfalloons.
- Very few people know who Foghorn Leghorn is.
