Keasling: Synthetic Biology Class/Questions

Questions for Engineering E. coli to see light:

1. What is the purpose of the system? What are the "parts" of the system? What are the inputs and outputs of each part? What are the inputs and outputs of the overall system? How could you use each of the "parts" for other applications?

Questions for Engineering a mevalonate pathway in E. coli for production of terpenoids:

2. What is the purpose of the system? What are the "parts" of the system designed? What are the inputs and outputs of each part? What are the inputs and outputs of the overall system?

3. What are the advantages of splitting the biosynthetic genes into multiple operons? What could be the disadvantages?

4. What were some of the problems encountered while engineering E. coli to produce amorphadiene? How did the authors overcome those problems? How was the system optimized for production of amorphadiene in E. coli?

5. What other types of applications could you build based on this system?