Keck Subbasement:Equipment

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Available equipement

Rough list of equipment which is currently available (and used for teaching)

PCR machine Gel equipment Several microscopes (schedule via Google Calendar) Small centrifuge + tiny centrifuge Miscellaneous equipment: pipetters

Fume hood Pelton and Crane sterilizer - small, not sure if it is working

There is also some equipment in nearby instructional labs (mainly Spalding) that we can use over the summer

• Med/large centrifuge - Spalding (purchased by RMM in summer 207)
• Electroporation machine - Spalding or Broad (not sure where)
• Plate reader - Spalding
• -80 freezer space - Spalding

Missing equipment

Here is a list of equipment that the lab is missing:

2008 list

• Balance ($3K)
• Autoclave - this can probably be shared with others in Spalding. A decent-sized autoclave looks like it costs $10-20K. Jared has one that is out for repair. Chinlin does not currently have an autoclave
• Spectroflourimeter ($25K+)
• Spectrophotometer ($??) or nanodrop ($8K) - share with others in Spalding. Check on current spectrophotometer first
• Nanopure (water filtration machine) - a small benchtop unit seems to cost about $3-5K
• Hot plate with stirrer (found one in the lab)
• Ice machine - the one in the subbasement is broken. Jared has one; Chinlin has ordered one => we can probably do without
• Gel imager ($8-10K) - we have the equipment required to image gels by hand; might be useful at some point to get a real imager

2007 list

• Electroporation Machine
• Filters for scope that can see mCherry, cerulean, and YFP
• -80 deg C freezer - available in Spalding
• Fluorimeter - available in Spalding, but might be nice to have one
• Equipment for running polyacrylamide gels

Purchased

• Medium/Large Centrifuge (for minipreps, competent cells, falcon tubes) - purchased in 2007; now sitting in the Spalding instructional laboratory