Keck Subbasement:Inventory Aug 2010: Difference between revisions
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(New page: This inventory is intended to give a full accounting of durable objects in Keck 040 and a general sense for what consumables are available in Keck, conducted on Aug. 18 by JTM. == Norther...) |
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== Northern Bench == | == Northern Bench == | ||
* | * Equipment | ||
* | ** Eppendorf Centrifuge 5810R | ||
*** Rotor for 30 small 1-2mL tubes | |||
*** Large rotor with inserts up to 5x 50mL tube | |||
*** Tools for changing out rotor etc. in cabinets below | |||
** Pelton & Crane Small Autoclave (working?) | |||
** Millipore Direct-Q 3 water purifier | |||
** Alarm clock | |||
** Eppendorf Vacufuge Plus | |||
** 2x sets of Eppendorf reference pipettes | |||
** Tiny benchtop centrifuge | |||
** Bio-Rad BR-2000 vortexer | |||
** Bio-Rad MJ Mini PCR thermocycler w/ 48 well block (can do thermal gradients) | |||
** Thermo Scientific Nanodrop 2000C | |||
** Elmer-Perkin Victor X3 2030 Multilabel Plate reader + Dell Vostro 1000 Laptop | |||
** Freezer | |||
* Chemicals & Kits | |||
** Qiagen PCR Purification Kits | |||
** Promega Wizard Plus SV Miniprep kit | |||
** Ultrapure Agarose | |||
** Glycerol | |||
** Urea | |||
** Ammonium Persulfate | |||
** LiCl | |||
** MgCl * 6 H2O | |||
** EtBr | |||
** Ethylene Glycol | |||
** Malachite Green oxalate | |||
** KCl | |||
** Mg acetate * 4 H20 | |||
** Tris-EDTA 10x | |||
** Theophylline | |||
* Misc | |||
** Pipette tips, falcon tubes, eppendorf tubes, and 96 well plates | |||
** Paraflim | |||
** Sink, drying racks, detergents etc. | |||
** Fluorimeter manuals, quartz cuvettes, cleaning supplies, and replacement lamps | |||
** Scale manual, accessories, weighing boats | |||
** Box of 15 mL glass tubes with plastic tops | |||
** Binders, pens | |||
== Western Bench == | == Western Bench == | ||
* | * Equipment | ||
* | ** Bio-Rad BR-2000 Vortexer | ||
** Corning PC-420D hot plate / stirrer | |||
** Tiny benchtop centrifuge | |||
** VWR Standard Heatblock (fits 24 eppendorf tubes) | |||
** Fisher Scientific Isotemp 2150 Nano | |||
** UV lamp (365 nm & white light) | |||
** Mettler-Toledo AB54-S/Fact Scale (10mg to 50g) | |||
** Pipette + charger | |||
** Labnet International Enduro Power Supply 300V | |||
** Fisher Scientific FB300 Power Supply | |||
** Two large gel boxes + four gel trays + many combs | |||
** Cell SureLock PAGE gel stand | |||
** Chemical Hood | |||
** Fridge | |||
** Tilt Box | |||
** Unknown pump (looks like its an aquarium pump) | |||
** Unknown glass/metal tube (four attachments, gas tube sealed in metal frame, possibly electrical linkages on top?) | |||
** Vacuum pump (under chemical hood) | |||
* Chemicals & Kits | |||
** 10X TBE | |||
** 10X TAE | |||
** 200 proof EtOH USP grade (in cabinet under hood) | |||
** 1x Bonner's Salt | |||
** anamycin sulfate | |||
** 4x 2008 miniprep kits | |||
2x gel extraction kits | |||
* Misc | |||
** Al foil | |||
** Gloves | |||
** Pipettes (10ml to 100ml) | |||
** Saran wrap | |||
== Southern Wall == | == Southern Wall == | ||
* Equipment | |||
** Microwave | |||
** Empty cabinet with gas tank attachments | |||
** Alpha Innotech Mini Gel Imager (302nm & 365nm) + Thermal printer & computer | |||
** Thermo Scientific Revco Value Plus -80C | |||
** Dell 17" LCD screen, unattached to a computer | |||
** Xerox Phaser 6100 Printer (dsdna) | |||
** Toolkit with wrenches & tubing | |||
** Three inkjet printers, unconnected to anything | |||
== Fluorimeter Room == | == Fluorimeter Room == | ||
* Equipment | |||
** Horibajobinyvon Fluorolog with computer, thermal management | |||
** Bio-Rad BR-2000 Vortexer | |||
** Tiny benchtop centrifuge | |||
** Large empty crate | |||
* Chemicals | |||
** n-Hexadecane | |||
** Silicone oil | |||
== The Island == | == The Island == | ||
* Equipment | |||
** Pixma MP210 Scanner/Printer | |||
** UV Faceshield, good condition | |||
** Thermo Scientific MaxQ Shaker (heating only) | |||
** Eppendorf 5418 Centrifuge with 18 eppendorf rotor | |||
** Power outlet from ceiling | |||
== The Freezer == | == The Freezer == | ||
** EtOH | |||
** rhodamine phalloidin | |||
** Adenosine-3', 5'cyclic monophosphate sodium salt | |||
** 2x Monoclonal antitubulin polyglutmylated antibody | |||
** 2x Taq polymerase (expiring 6/2011) | |||
** DAPI | |||
** Sybr Gold | |||
** 1kb and 100bp ladders | |||
** Phusion sample (assayed 07/09 - possibly empty) | |||
** Phire Hot Start PCR Sample (assayed 07/09 - almost empty) | |||
** Glycogen (Ambion) | |||
** 50x gel buffer II tubes (Ambion | |||
** T7 RNA Polymerase 300k U | |||
** 8x PCR dNTP mix 10mM (expiring 9/2010) | |||
** Many tubes of TURBO DNase (in MEGAshortscript T7 boxes, dated 4/22/09, 6/15/09, 8/14/09, and 10/23/09 respectively) | |||
** 2x MEGAshortscript T7 boxes | |||
** Cold packs | |||
** Plastic tube rack with 25 cryptically labeled tubes of clear fluid/nothing | |||
** Blue Keep-Cold Freezer Box | |||
*** Pureexpress solutions | |||
*** RNase H | |||
*** Pyrophosphatase | |||
*** Ribonuclease R | |||
*** T4 DNA Ligase (expiring 7/2010) | |||
*** T4 DNA Ligase buffer (expiring 8/2011) | |||
*** T7 enzyme mixes | |||
*** T7 RNA Polymerase | |||
** Bags of tubes | |||
*** "Arjun's Plasmids" | |||
*** "EPIBIO Buf & DTT 5-2010" | |||
*** "Elisa EPIBIO TXN KIT STOCK 6-10-2009" | |||
*** Tubes of DNA from IDT to "P. Forsburg" | |||
** Freezer Boxes | |||
*** "Elisa PFRR 2008-2010" | |||
*** "FEI" | |||
*** "Elisa Franco" | |||
*** "Elisa TXN" | |||
*** "Bistable circuit" | |||
*** "EF Rybozymes" | |||
*** "Elisa oscillator for patterns" | |||
*** "RNA Elisa" | |||
*** "Elisa NFRR V2" | |||
*** "DNA for beads / tweezers Elisa 9/24/2008 8/25/2009" | |||
*** "Roman DNA-box" | |||
*** "EF OSC SEP 09" | |||
*** Unlabeled with many multicolored tubes | |||
*** Unlabeled with few tubes with loading buffer colored droplets in them | |||
== The Fridge == | == The Fridge == | ||
* 38% acrylamide/2% bis stock (Ambion) | |||
* Anti-mouse IgM FITC (targets /mu chains) | |||
* TEMED | |||
* Precast HEPES & TBE gels | |||
* Bradford assay reagent (no protein standards found to go with) | |||
* Unlabeled silver canister, tightly sealed, bagged, taped, and cable-tied | |||
* Two empty styrofoam boxes | |||
* One possibly unopened styrofoam box from Invitrogen to Elisa Franco | |||
* Hoechst 33258 pentahydrate bis-benzamide |
Revision as of 13:07, 19 August 2010
This inventory is intended to give a full accounting of durable objects in Keck 040 and a general sense for what consumables are available in Keck, conducted on Aug. 18 by JTM.
Northern Bench
- Equipment
- Eppendorf Centrifuge 5810R
- Rotor for 30 small 1-2mL tubes
- Large rotor with inserts up to 5x 50mL tube
- Tools for changing out rotor etc. in cabinets below
- Pelton & Crane Small Autoclave (working?)
- Millipore Direct-Q 3 water purifier
- Alarm clock
- Eppendorf Vacufuge Plus
- 2x sets of Eppendorf reference pipettes
- Tiny benchtop centrifuge
- Bio-Rad BR-2000 vortexer
- Bio-Rad MJ Mini PCR thermocycler w/ 48 well block (can do thermal gradients)
- Thermo Scientific Nanodrop 2000C
- Elmer-Perkin Victor X3 2030 Multilabel Plate reader + Dell Vostro 1000 Laptop
- Freezer
- Eppendorf Centrifuge 5810R
- Chemicals & Kits
- Qiagen PCR Purification Kits
- Promega Wizard Plus SV Miniprep kit
- Ultrapure Agarose
- Glycerol
- Urea
- Ammonium Persulfate
- LiCl
- MgCl * 6 H2O
- EtBr
- Ethylene Glycol
- Malachite Green oxalate
- KCl
- Mg acetate * 4 H20
- Tris-EDTA 10x
- Theophylline
- Misc
- Pipette tips, falcon tubes, eppendorf tubes, and 96 well plates
- Paraflim
- Sink, drying racks, detergents etc.
- Fluorimeter manuals, quartz cuvettes, cleaning supplies, and replacement lamps
- Scale manual, accessories, weighing boats
- Box of 15 mL glass tubes with plastic tops
- Binders, pens
Western Bench
- Equipment
- Bio-Rad BR-2000 Vortexer
- Corning PC-420D hot plate / stirrer
- Tiny benchtop centrifuge
- VWR Standard Heatblock (fits 24 eppendorf tubes)
- Fisher Scientific Isotemp 2150 Nano
- UV lamp (365 nm & white light)
- Mettler-Toledo AB54-S/Fact Scale (10mg to 50g)
- Pipette + charger
- Labnet International Enduro Power Supply 300V
- Fisher Scientific FB300 Power Supply
- Two large gel boxes + four gel trays + many combs
- Cell SureLock PAGE gel stand
- Chemical Hood
- Fridge
- Tilt Box
- Unknown pump (looks like its an aquarium pump)
- Unknown glass/metal tube (four attachments, gas tube sealed in metal frame, possibly electrical linkages on top?)
- Vacuum pump (under chemical hood)
- Chemicals & Kits
- 10X TBE
- 10X TAE
- 200 proof EtOH USP grade (in cabinet under hood)
- 1x Bonner's Salt
- anamycin sulfate
- 4x 2008 miniprep kits
2x gel extraction kits
- Misc
- Al foil
- Gloves
- Pipettes (10ml to 100ml)
- Saran wrap
Southern Wall
- Equipment
- Microwave
- Empty cabinet with gas tank attachments
- Alpha Innotech Mini Gel Imager (302nm & 365nm) + Thermal printer & computer
- Thermo Scientific Revco Value Plus -80C
- Dell 17" LCD screen, unattached to a computer
- Xerox Phaser 6100 Printer (dsdna)
- Toolkit with wrenches & tubing
- Three inkjet printers, unconnected to anything
Fluorimeter Room
- Equipment
- Horibajobinyvon Fluorolog with computer, thermal management
- Bio-Rad BR-2000 Vortexer
- Tiny benchtop centrifuge
- Large empty crate
- Chemicals
- n-Hexadecane
- Silicone oil
The Island
- Equipment
- Pixma MP210 Scanner/Printer
- UV Faceshield, good condition
- Thermo Scientific MaxQ Shaker (heating only)
- Eppendorf 5418 Centrifuge with 18 eppendorf rotor
- Power outlet from ceiling
The Freezer
- EtOH
- rhodamine phalloidin
- Adenosine-3', 5'cyclic monophosphate sodium salt
- 2x Monoclonal antitubulin polyglutmylated antibody
- 2x Taq polymerase (expiring 6/2011)
- DAPI
- Sybr Gold
- 1kb and 100bp ladders
- Phusion sample (assayed 07/09 - possibly empty)
- Phire Hot Start PCR Sample (assayed 07/09 - almost empty)
- Glycogen (Ambion)
- 50x gel buffer II tubes (Ambion
- T7 RNA Polymerase 300k U
- 8x PCR dNTP mix 10mM (expiring 9/2010)
- Many tubes of TURBO DNase (in MEGAshortscript T7 boxes, dated 4/22/09, 6/15/09, 8/14/09, and 10/23/09 respectively)
- 2x MEGAshortscript T7 boxes
- Cold packs
- Plastic tube rack with 25 cryptically labeled tubes of clear fluid/nothing
- Blue Keep-Cold Freezer Box
- Pureexpress solutions
- RNase H
- Pyrophosphatase
- Ribonuclease R
- T4 DNA Ligase (expiring 7/2010)
- T4 DNA Ligase buffer (expiring 8/2011)
- T7 enzyme mixes
- T7 RNA Polymerase
- Blue Keep-Cold Freezer Box
- Bags of tubes
- "Arjun's Plasmids"
- "EPIBIO Buf & DTT 5-2010"
- "Elisa EPIBIO TXN KIT STOCK 6-10-2009"
- Tubes of DNA from IDT to "P. Forsburg"
- Bags of tubes
- Freezer Boxes
- "Elisa PFRR 2008-2010"
- "FEI"
- "Elisa Franco"
- "Elisa TXN"
- "Bistable circuit"
- "EF Rybozymes"
- "Elisa oscillator for patterns"
- "RNA Elisa"
- "Elisa NFRR V2"
- "DNA for beads / tweezers Elisa 9/24/2008 8/25/2009"
- "Roman DNA-box"
- "EF OSC SEP 09"
- Unlabeled with many multicolored tubes
- Unlabeled with few tubes with loading buffer colored droplets in them
- Freezer Boxes
The Fridge
- 38% acrylamide/2% bis stock (Ambion)
- Anti-mouse IgM FITC (targets /mu chains)
- TEMED
- Precast HEPES & TBE gels
- Bradford assay reagent (no protein standards found to go with)
- Unlabeled silver canister, tightly sealed, bagged, taped, and cable-tied
- Two empty styrofoam boxes
- One possibly unopened styrofoam box from Invitrogen to Elisa Franco
- Hoechst 33258 pentahydrate bis-benzamide