Keck Subbasement:Inventory Aug 2010

This inventory is intended to give a full accounting of durable objects in Keck 040 and a general sense for what consumables are available in Keck, conducted on Aug. 18 by JTM. Other inventories are / will be listed here.

Northern Bench

• Equipment
  • Eppendorf Centrifuge 5810R
    • Rotor for 30 small 1-2mL tubes
    • Large rotor with inserts up to 5x 50mL tube
    • Tools for changing out rotor etc. in cabinets below
  • Pelton & Crane Small Autoclave (working?)
  • Millipore Direct-Q 3 water purifier
  • Alarm clock
  • Eppendorf Vacufuge Plus
  • 2x sets of Eppendorf reference pipettes
  • Tiny benchtop centrifuge
  • Bio-Rad BR-2000 vortexer
  • Bio-Rad MJ Mini PCR thermocycler w/ 48 well block (can do thermal gradients)
  • Thermo Scientific Nanodrop 2000C
  • Elmer-Perkin Victor X3 2030 Multilabel Plate reader + Dell Vostro 1000 Laptop
  • Small Freezer
  • 3x 500 mL glass bottles with caps
  • 2x 250 mL erlenmeyer flasks
  • Pipette tips, falcon tubes, eppendorf tubes, and 96 well plates
  • Paraflim
  • Sink, drying racks, detergents etc.
  • Fluorimeter manuals, quartz cuvettes, cleaning supplies, and replacement lamps
  • Scale manual, accessories, weighing boats
  • Box of 15 mL glass tubes with plastic tops
  • Binders, pens

• Chemicals & Kits
  • Qiagen PCR Purification Kits
  • Promega Wizard Plus SV Miniprep kit
  • Ultrapure Agarose
  • Glycerol
  • Urea
  • Ammonium Persulfate
  • LiCl
  • MgCl * 6 H2O
  • EtBr
  • Ethylene Glycol
  • Malachite Green oxalate
  • KCl
  • Mg acetate * 4 H20
  • Tris-EDTA 10x
  • Theophylline

Western Bench

• Equipment
  • Bio-Rad BR-2000 Vortexer
  • Corning PC-420D hot plate / stirrer
  • Tiny benchtop centrifuge
  • VWR Standard Heatblock (fits 24 eppendorf tubes)
  • Fisher Scientific Isotemp 2150 Nano
  • UV lamp (365 nm & white light)
  • Mettler-Toledo AB54-S/Fact Scale (10mg to 50g)
  • Pipette + charger
  • Labnet International Enduro Power Supply 300V
  • Fisher Scientific FB300 Power Supply
  • Two large gel boxes + four gel trays + many combs
  • Cell SureLock PAGE gel stand
  • Chemical Hood
  • Fridge
  • Tilt Box
  • Unknown pump (looks like its an aquarium pump)
  • Unknown glass/metal tube (four attachments, gas tube sealed in metal frame, possibly electrical linkages on top?)
  • Vacuum pump (under chemical hood)

• Chemicals & Kits
  • 2x boxes of Qiagen gel extraction kits, not dated
  • 4x boxes of Qiagen miniprep kits, two dated to 2008
  • 10X TBE
  • 10X TAE
  • 200 proof EtOH USP grade (in cabinet under hood)
  • 1x Bonner's Salt
  • kanamycin sulfate

• Misc
  • Al foil
  • Gloves
  • Pipettes (10ml to 100ml)
  • Saran wrap

Southern Wall

• Equipment
  • Microwave
  • Empty cabinet with gas tank attachments
  • Alpha Innotech Mini Gel Imager (302nm & 365nm) + Thermal printer & computer
  • Thermo Scientific Revco Value Plus -80C
  • Dell 17" LCD screen, unattached to a computer
  • Xerox Phaser 6100 Printer (dsdna)
  • Toolkit with wrenches & tubing
  • Three inkjet printers, unconnected to anything

Fluorimeter Room

• Equipment
  • Horibajobinyvon Fluorolog 3 with computer, thermal management
  • Bio-Rad BR-2000 Vortexer
  • Tiny benchtop centrifuge
  • Large empty crate

• Chemicals
  • n-Hexadecane
  • Silicone oil

The Island

• Equipment
  • Pixma MP210 Scanner/Printer
  • UV Faceshield, good condition
  • Thermo Scientific MaxQ Shaker (heating only)
  • Eppendorf 5418 Centrifuge with 18 eppendorf rotor
  • Power outlet from ceiling

The Freezer

• EtOH
• rhodamine phalloidin
• Adenosine-3', 5'cyclic monophosphate sodium salt
• 2x Monoclonal antitubulin polyglutmylated antibody
• 2x Taq polymerase (expiring 6/2011)
• DAPI
• Sybr Gold
• 1kb and 100bp ladders
• Phusion sample (assayed 07/09 - possibly empty)
• Phire Hot Start PCR Sample (assayed 07/09 - almost empty)
• Glycogen (Ambion)
• 50x gel buffer II tubes (Ambion
• T7 RNA Polymerase 300k U
• 8x PCR dNTP mix 10mM (expiring 9/2010)
• Many tubes of TURBO DNase (in MEGAshortscript T7 boxes, dated 4/22/09, 6/15/09, 8/14/09, and 10/23/09 respectively)
• 2x MEGAshortscript T7 boxes
• Cold packs
• Plastic tube rack with 25 cryptically labeled tubes of clear fluid/nothing
• Blue Keep-Cold Freezer Box
  • Pureexpress solutions
  • RNase H
  • Pyrophosphatase
  • Ribonuclease R
  • T4 DNA Ligase (expiring 7/2010)
  • T4 DNA Ligase buffer (expiring 8/2011)
  • T7 enzyme mixes
  • T7 RNA Polymerase
• Bags of tubes
  • "Arjun's Plasmids"
  • "EPIBIO Buf & DTT 5-2010"
  • "Elisa EPIBIO TXN KIT STOCK 6-10-2009"
  • Tubes of DNA from IDT to "P. Forsburg"

• Freezer Boxes
  • "Elisa PFRR 2008-2010"
  • "FEI"
  • "Elisa Franco"
  • "Elisa TXN"
  • "Bistable circuit"
  • "EF Rybozymes"
  • "Elisa oscillator for patterns"
  • "RNA Elisa"
  • "Elisa NFRR V2"
  • "DNA for beads / tweezers Elisa 9/24/2008 8/25/2009"
  • "Roman DNA-box"
  • "EF OSC SEP 09"
  • Unlabeled with many multicolored tubes
  • Unlabeled with a few tubes with loading buffer colored droplets in them

The Fridge

• 38% acrylamide/2% bis stock (Ambion)
• Anti-mouse IgM FITC (targets /mu chains)
• TEMED
• Precast HEPES & TBE gels
• Bradford assay reagent (no protein standards found to go with)
• Unlabeled silver canister, tightly sealed, bagged, taped, and cable-tied
• Two empty styrofoam boxes
• One possibly unopened styrofoam box from Invitrogen to Elisa Franco
• Hoechst 33258 pentahydrate bis-benzamide

Ophelia's equipment & molecular biology reagents in Broad

• Equipment
  • Refrigerated shaker (basement of Broad, Rm 40)
  • Temperature block
  • Gilson pipetmen (P2, 20, 100, 200, 1000)
  • Galaxy ministar centrifuge (for 1.5 ml and PCR tubes)

• Restriction enzymes
  • AgeI
  • ApaI
  • AscI
  • BamHI & BamHI-HF
  • 2*BbsI (not stable for longer than 30 days at -20C-->according to NEB, check if still functional)
  • BstXI
  • Bsu36I
  • CIP
  • DpnI
  • EcoRI & EcoRI-HF
  • KpnI
  • NotI
  • NruI
  • PacI
  • PspOMI
  • SacI-HF
  • SalI-HF
  • XbaI
  • XhoI

• PCR enzymes & reagents
  • small amount of dNTPs
  • T4 DNA Ligase
  • Platinum Taq
  • Taq
  • Pfx

• Small molecules
  • Arabinose
  • aTc
  • Doxycycline
  • IPTG

• Antibiotics
  • Kanamycin sulfate
  • Ampicillin
  • Chlorampenicol
  • Streptomycin
  • Geneticin (KAN)
  • Hygromycin (HPH)
  • Nourseothricin sulfate (NAT)

• Kits
  • Clonetech yeast transformation
  • Zymo research E coli competent cell
  • Zymo yeast genomic DNA isolation
  • Qiagen mini, PCR cleanup, gel purification
  • Quikchange kit for mutagenesis

• Sugars
  • Galactose
  • Glucose
  • Raffinose
  • Sucrose

• Liquid and plate media materials
  • Bacto Peptone
  • Bacto Yeast Extract
  • Bacto Agar

• Yeast transformation materials
  • salmon sperm DNA