Keck Subbasement:Inventory Aug 2010: Difference between revisions
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Line 224: | Line 224: | ||
** Ampicillin | ** Ampicillin | ||
** Chlorampenicol | ** Chlorampenicol | ||
** Streptomycin | |||
** Geneticin (KAN) | ** Geneticin (KAN) | ||
** Hygromycin (HPH) | ** Hygromycin (HPH) | ||
** Nourseothricin sulfate (NAT) | ** Nourseothricin sulfate (NAT) | ||
* Kits | * Kits |
Latest revision as of 16:35, 31 August 2010
This inventory is intended to give a full accounting of durable objects in Keck 040 and a general sense for what consumables are available in Keck, conducted on Aug. 18 by JTM. Other inventories are / will be listed here.
Northern Bench
- Equipment
- Eppendorf Centrifuge 5810R
- Rotor for 30 small 1-2mL tubes
- Large rotor with inserts up to 5x 50mL tube
- Tools for changing out rotor etc. in cabinets below
- Pelton & Crane Small Autoclave (working?)
- Millipore Direct-Q 3 water purifier
- Alarm clock
- Eppendorf Vacufuge Plus
- 2x sets of Eppendorf reference pipettes
- Tiny benchtop centrifuge
- Bio-Rad BR-2000 vortexer
- Bio-Rad MJ Mini PCR thermocycler w/ 48 well block (can do thermal gradients)
- Thermo Scientific Nanodrop 2000C
- Elmer-Perkin Victor X3 2030 Multilabel Plate reader + Dell Vostro 1000 Laptop
- Small Freezer
- 3x 500 mL glass bottles with caps
- 2x 250 mL erlenmeyer flasks
- Pipette tips, falcon tubes, eppendorf tubes, and 96 well plates
- Paraflim
- Sink, drying racks, detergents etc.
- Fluorimeter manuals, quartz cuvettes, cleaning supplies, and replacement lamps
- Scale manual, accessories, weighing boats
- Box of 15 mL glass tubes with plastic tops
- Binders, pens
- Eppendorf Centrifuge 5810R
- Chemicals & Kits
- Qiagen PCR Purification Kits
- Promega Wizard Plus SV Miniprep kit
- Ultrapure Agarose
- Glycerol
- Urea
- Ammonium Persulfate
- LiCl
- MgCl * 6 H2O
- EtBr
- Ethylene Glycol
- Malachite Green oxalate
- KCl
- Mg acetate * 4 H20
- Tris-EDTA 10x
- Theophylline
Western Bench
- Equipment
- Bio-Rad BR-2000 Vortexer
- Corning PC-420D hot plate / stirrer
- Tiny benchtop centrifuge
- VWR Standard Heatblock (fits 24 eppendorf tubes)
- Fisher Scientific Isotemp 2150 Nano
- UV lamp (365 nm & white light)
- Mettler-Toledo AB54-S/Fact Scale (10mg to 50g)
- Pipette + charger
- Labnet International Enduro Power Supply 300V
- Fisher Scientific FB300 Power Supply
- Two large gel boxes + four gel trays + many combs
- Cell SureLock PAGE gel stand
- Chemical Hood
- Fridge
- Tilt Box
- Unknown pump (looks like its an aquarium pump)
- Unknown glass/metal tube (four attachments, gas tube sealed in metal frame, possibly electrical linkages on top?)
- Vacuum pump (under chemical hood)
- Chemicals & Kits
- 2x boxes of Qiagen gel extraction kits, not dated
- 4x boxes of Qiagen miniprep kits, two dated to 2008
- 10X TBE
- 10X TAE
- 200 proof EtOH USP grade (in cabinet under hood)
- 1x Bonner's Salt
- kanamycin sulfate
- Misc
- Al foil
- Gloves
- Pipettes (10ml to 100ml)
- Saran wrap
Southern Wall
- Equipment
- Microwave
- Empty cabinet with gas tank attachments
- Alpha Innotech Mini Gel Imager (302nm & 365nm) + Thermal printer & computer
- Thermo Scientific Revco Value Plus -80C
- Dell 17" LCD screen, unattached to a computer
- Xerox Phaser 6100 Printer (dsdna)
- Toolkit with wrenches & tubing
- Three inkjet printers, unconnected to anything
Fluorimeter Room
- Equipment
- Horibajobinyvon Fluorolog 3 with computer, thermal management
- Bio-Rad BR-2000 Vortexer
- Tiny benchtop centrifuge
- Large empty crate
- Chemicals
- n-Hexadecane
- Silicone oil
The Island
- Equipment
- Pixma MP210 Scanner/Printer
- UV Faceshield, good condition
- Thermo Scientific MaxQ Shaker (heating only)
- Eppendorf 5418 Centrifuge with 18 eppendorf rotor
- Power outlet from ceiling
The Freezer
- EtOH
- rhodamine phalloidin
- Adenosine-3', 5'cyclic monophosphate sodium salt
- 2x Monoclonal antitubulin polyglutmylated antibody
- 2x Taq polymerase (expiring 6/2011)
- DAPI
- Sybr Gold
- 1kb and 100bp ladders
- Phusion sample (assayed 07/09 - possibly empty)
- Phire Hot Start PCR Sample (assayed 07/09 - almost empty)
- Glycogen (Ambion)
- 50x gel buffer II tubes (Ambion
- T7 RNA Polymerase 300k U
- 8x PCR dNTP mix 10mM (expiring 9/2010)
- Many tubes of TURBO DNase (in MEGAshortscript T7 boxes, dated 4/22/09, 6/15/09, 8/14/09, and 10/23/09 respectively)
- 2x MEGAshortscript T7 boxes
- Cold packs
- Plastic tube rack with 25 cryptically labeled tubes of clear fluid/nothing
- Blue Keep-Cold Freezer Box
- Pureexpress solutions
- RNase H
- Pyrophosphatase
- Ribonuclease R
- T4 DNA Ligase (expiring 7/2010)
- T4 DNA Ligase buffer (expiring 8/2011)
- T7 enzyme mixes
- T7 RNA Polymerase
- Bags of tubes
- "Arjun's Plasmids"
- "EPIBIO Buf & DTT 5-2010"
- "Elisa EPIBIO TXN KIT STOCK 6-10-2009"
- Tubes of DNA from IDT to "P. Forsburg"
- Freezer Boxes
- "Elisa PFRR 2008-2010"
- "FEI"
- "Elisa Franco"
- "Elisa TXN"
- "Bistable circuit"
- "EF Rybozymes"
- "Elisa oscillator for patterns"
- "RNA Elisa"
- "Elisa NFRR V2"
- "DNA for beads / tweezers Elisa 9/24/2008 8/25/2009"
- "Roman DNA-box"
- "EF OSC SEP 09"
- Unlabeled with many multicolored tubes
- Unlabeled with a few tubes with loading buffer colored droplets in them
The Fridge
- 38% acrylamide/2% bis stock (Ambion)
- Anti-mouse IgM FITC (targets /mu chains)
- TEMED
- Precast HEPES & TBE gels
- Bradford assay reagent (no protein standards found to go with)
- Unlabeled silver canister, tightly sealed, bagged, taped, and cable-tied
- Two empty styrofoam boxes
- One possibly unopened styrofoam box from Invitrogen to Elisa Franco
- Hoechst 33258 pentahydrate bis-benzamide
Ophelia's equipment & molecular biology reagents in Broad
- Equipment
- Refrigerated shaker (basement of Broad, Rm 40)
- Temperature block
- Gilson pipetmen (P2, 20, 100, 200, 1000)
- Galaxy ministar centrifuge (for 1.5 ml and PCR tubes)
- Restriction enzymes
- AgeI
- ApaI
- AscI
- BamHI & BamHI-HF
- 2*BbsI (not stable for longer than 30 days at -20C-->according to NEB, check if still functional)
- BstXI
- Bsu36I
- CIP
- DpnI
- EcoRI & EcoRI-HF
- KpnI
- NotI
- NruI
- PacI
- PspOMI
- SacI-HF
- SalI-HF
- XbaI
- XhoI
- PCR enzymes & reagents
- small amount of dNTPs
- T4 DNA Ligase
- Platinum Taq
- Taq
- Pfx
- Small molecules
- Arabinose
- aTc
- Doxycycline
- IPTG
- Antibiotics
- Kanamycin sulfate
- Ampicillin
- Chlorampenicol
- Streptomycin
- Geneticin (KAN)
- Hygromycin (HPH)
- Nourseothricin sulfate (NAT)
- Kits
- Clonetech yeast transformation
- Zymo research E coli competent cell
- Zymo yeast genomic DNA isolation
- Qiagen mini, PCR cleanup, gel purification
- Quikchange kit for mutagenesis
- Sugars
- Galactose
- Glucose
- Raffinose
- Sucrose
- Liquid and plate media materials
- Bacto Peptone
- Bacto Yeast Extract
- Bacto Agar
- Yeast transformation materials
- salmon sperm DNA