Keck Subbasement:Inventory Aug 2010

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(Ophelia's Equipment & molecular biology reagents in Broad)
(Ophelia's Equipment & molecular biology reagents in Broad)
Line 227: Line 227:
** Hygromycin (HPH)
** Hygromycin (HPH)
** Nourseothricin sulfate (NAT)
** Nourseothricin sulfate (NAT)
 +
** Streptomycin
* Kits
* Kits
Line 234: Line 235:
** Qiagen mini, PCR cleanup, gel purification
** Qiagen mini, PCR cleanup, gel purification
** Quikchange kit for mutagenesis
** Quikchange kit for mutagenesis
 +
 +
* Sugars
 +
** Galactose
 +
** Glucose
 +
** Raffinose
 +
** Sucrose
* Yeast transformation materials
* Yeast transformation materials
** salmon sperm DNA
** salmon sperm DNA

Revision as of 17:37, 31 August 2010

This inventory is intended to give a full accounting of durable objects in Keck 040 and a general sense for what consumables are available in Keck, conducted on Aug. 18 by JTM. Other inventories are / will be listed here.

Contents

Northern Bench

  • Equipment
    • Eppendorf Centrifuge 5810R
      • Rotor for 30 small 1-2mL tubes
      • Large rotor with inserts up to 5x 50mL tube
      • Tools for changing out rotor etc. in cabinets below
    • Pelton & Crane Small Autoclave (working?)
    • Millipore Direct-Q 3 water purifier
    • Alarm clock
    • Eppendorf Vacufuge Plus
    • 2x sets of Eppendorf reference pipettes
    • Tiny benchtop centrifuge
    • Bio-Rad BR-2000 vortexer
    • Bio-Rad MJ Mini PCR thermocycler w/ 48 well block (can do thermal gradients)
    • Thermo Scientific Nanodrop 2000C
    • Elmer-Perkin Victor X3 2030 Multilabel Plate reader + Dell Vostro 1000 Laptop
    • Small Freezer
    • 3x 500 mL glass bottles with caps
    • 2x 250 mL erlenmeyer flasks
    • Pipette tips, falcon tubes, eppendorf tubes, and 96 well plates
    • Paraflim
    • Sink, drying racks, detergents etc.
    • Fluorimeter manuals, quartz cuvettes, cleaning supplies, and replacement lamps
    • Scale manual, accessories, weighing boats
    • Box of 15 mL glass tubes with plastic tops
    • Binders, pens
  • Chemicals & Kits
    • Qiagen PCR Purification Kits
    • Promega Wizard Plus SV Miniprep kit
    • Ultrapure Agarose
    • Glycerol
    • Urea
    • Ammonium Persulfate
    • LiCl
    • MgCl * 6 H2O
    • EtBr
    • Ethylene Glycol
    • Malachite Green oxalate
    • KCl
    • Mg acetate * 4 H20
    • Tris-EDTA 10x
    • Theophylline

Western Bench

  • Equipment
    • Bio-Rad BR-2000 Vortexer
    • Corning PC-420D hot plate / stirrer
    • Tiny benchtop centrifuge
    • VWR Standard Heatblock (fits 24 eppendorf tubes)
    • Fisher Scientific Isotemp 2150 Nano
    • UV lamp (365 nm & white light)
    • Mettler-Toledo AB54-S/Fact Scale (10mg to 50g)
    • Pipette + charger
    • Labnet International Enduro Power Supply 300V
    • Fisher Scientific FB300 Power Supply
    • Two large gel boxes + four gel trays + many combs
    • Cell SureLock PAGE gel stand
    • Chemical Hood
    • Fridge
    • Tilt Box
    • Unknown pump (looks like its an aquarium pump)
    • Unknown glass/metal tube (four attachments, gas tube sealed in metal frame, possibly electrical linkages on top?)
    • Vacuum pump (under chemical hood)
  • Chemicals & Kits
    • 2x boxes of Qiagen gel extraction kits, not dated
    • 4x boxes of Qiagen miniprep kits, two dated to 2008
    • 10X TBE
    • 10X TAE
    • 200 proof EtOH USP grade (in cabinet under hood)
    • 1x Bonner's Salt
    • kanamycin sulfate
  • Misc
    • Al foil
    • Gloves
    • Pipettes (10ml to 100ml)
    • Saran wrap

Southern Wall

  • Equipment
    • Microwave
    • Empty cabinet with gas tank attachments
    • Alpha Innotech Mini Gel Imager (302nm & 365nm) + Thermal printer & computer
    • Thermo Scientific Revco Value Plus -80C
    • Dell 17" LCD screen, unattached to a computer
    • Xerox Phaser 6100 Printer (dsdna)
    • Toolkit with wrenches & tubing
    • Three inkjet printers, unconnected to anything

Fluorimeter Room

  • Equipment
    • Horibajobinyvon Fluorolog 3 with computer, thermal management
    • Bio-Rad BR-2000 Vortexer
    • Tiny benchtop centrifuge
    • Large empty crate
  • Chemicals
    • n-Hexadecane
    • Silicone oil

The Island

  • Equipment
    • Pixma MP210 Scanner/Printer
    • UV Faceshield, good condition
    • Thermo Scientific MaxQ Shaker (heating only)
    • Eppendorf 5418 Centrifuge with 18 eppendorf rotor
    • Power outlet from ceiling

The Freezer

  • EtOH
  • rhodamine phalloidin
  • Adenosine-3', 5'cyclic monophosphate sodium salt
  • 2x Monoclonal antitubulin polyglutmylated antibody
  • 2x Taq polymerase (expiring 6/2011)
  • DAPI
  • Sybr Gold
  • 1kb and 100bp ladders
  • Phusion sample (assayed 07/09 - possibly empty)
  • Phire Hot Start PCR Sample (assayed 07/09 - almost empty)
  • Glycogen (Ambion)
  • 50x gel buffer II tubes (Ambion
  • T7 RNA Polymerase 300k U
  • 8x PCR dNTP mix 10mM (expiring 9/2010)
  • Many tubes of TURBO DNase (in MEGAshortscript T7 boxes, dated 4/22/09, 6/15/09, 8/14/09, and 10/23/09 respectively)
  • 2x MEGAshortscript T7 boxes
  • Cold packs
  • Plastic tube rack with 25 cryptically labeled tubes of clear fluid/nothing
  • Blue Keep-Cold Freezer Box
    • Pureexpress solutions
    • RNase H
    • Pyrophosphatase
    • Ribonuclease R
    • T4 DNA Ligase (expiring 7/2010)
    • T4 DNA Ligase buffer (expiring 8/2011)
    • T7 enzyme mixes
    • T7 RNA Polymerase
  • Bags of tubes
    • "Arjun's Plasmids"
    • "EPIBIO Buf & DTT 5-2010"
    • "Elisa EPIBIO TXN KIT STOCK 6-10-2009"
    • Tubes of DNA from IDT to "P. Forsburg"
  • Freezer Boxes
    • "Elisa PFRR 2008-2010"
    • "FEI"
    • "Elisa Franco"
    • "Elisa TXN"
    • "Bistable circuit"
    • "EF Rybozymes"
    • "Elisa oscillator for patterns"
    • "RNA Elisa"
    • "Elisa NFRR V2"
    • "DNA for beads / tweezers Elisa 9/24/2008 8/25/2009"
    • "Roman DNA-box"
    • "EF OSC SEP 09"
    • Unlabeled with many multicolored tubes
    • Unlabeled with a few tubes with loading buffer colored droplets in them

The Fridge

  • 38% acrylamide/2% bis stock (Ambion)
  • Anti-mouse IgM FITC (targets /mu chains)
  • TEMED
  • Precast HEPES & TBE gels
  • Bradford assay reagent (no protein standards found to go with)
  • Unlabeled silver canister, tightly sealed, bagged, taped, and cable-tied
  • Two empty styrofoam boxes
  • One possibly unopened styrofoam box from Invitrogen to Elisa Franco
  • Hoechst 33258 pentahydrate bis-benzamide

Ophelia's Equipment & molecular biology reagents in Broad

  • Equipment
    • Refrigerated shaker (basement of Broad, Rm 40)
    • Temperature block
    • Gilson pipetmen (P2, 20, 100, 200, 1000)
    • Galaxy ministar centrifuge (for 1.5 ml and PCR tubes)
  • Restriction enzymes
    • AgeI
    • ApaI
    • AscI
    • BamHI & BamHI-HF
    • 2*BbsI (not stable for longer than 30 days at -20C-->according to NEB, check if still functional)
    • BstXI
    • Bsu36I
    • CIP
    • DpnI
    • EcoRI & EcoRI-HF
    • KpnI
    • NotI
    • NruI
    • PacI
    • PspOMI
    • SacI-HF
    • SalI-HF
    • XbaI
    • XhoI
  • PCR enzymes & reagents
    • small amount of dNTPs
    • T4 DNA Ligase
    • Platinum Taq
    • Taq
    • Pfx
  • Small molecules
    • Arabinose
    • aTc
    • Doxycycline
    • IPTG
  • Antibiotics
    • Kanamycin sulfate
    • Ampicillin
    • Chlorampenicol
    • Geneticin (KAN)
    • Hygromycin (HPH)
    • Nourseothricin sulfate (NAT)
    • Streptomycin
  • Kits
    • Clonetech yeast transformation
    • Zymo research E coli competent cell
    • Zymo yeast genomic DNA isolation
    • Qiagen mini, PCR cleanup, gel purification
    • Quikchange kit for mutagenesis
  • Sugars
    • Galactose
    • Glucose
    • Raffinose
    • Sucrose
  • Yeast transformation materials
    • salmon sperm DNA
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